Figure 1 | Gene Therapy

Figure 1

From: Adenovirus-mediated artificial MicroRNAs targeting matrix or nucleoprotein genes protect mice against lethal influenza virus challenge

Figure 1

Screen for functional influenza-specific artificial microRNA. Both single (a) and chained (b) amiRNA candidates (1 μg) were transiently transfected into 293 T cells (plated at 1 × 105 per well in 24-well plate on the previous day), which were challenged 24 h later with 0.0005 MOI A/PR8 for screening by real-time PCR. pcDNA 6.2-GW/miR-neg transfectant and virus only served as controls, respectively. P<0.0001 for all amiRNAs compared with either controls. To assess the inhibitory effect on polymerase activity, indicated dose of NP-specific amiRNA plasmids along with mini-genome reporter assay-related plasmids were transfected into 293 T cells (c). Firefly luciferase activity were assayed in cell lysates and compared with Renilla activity 24 h later. Control group was normalized to 100%. NP-856, NP-969, M1-89+NP-856 and M1-89+NP-969 were in comparison with the control (P<0.05). All data were repeated in triplicate. One-way ANOVA was applied to compare with the control group.

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