Figure 3
Influenza targeting Ads protected ICR mice completely from lethal challenge of A/PR8. ICR mice (n=15) vaccinated with indicated Ads were challenged 24 h later with 5LD50 of A/PR8. Five mice from each group were euthanized and dissected 5 d.p.i. Left upper lung lobes were stained with hematoxylin and eosin (a) and scored for histological changes (b) (rAd(NP-856), P=0.011, rAd(M1-89+NP-856), P=0.001, others, P<0.0001). Sections were representative of each group. Lung viral copy numbers were determined by absolute quantification RT-PCR (c) (rAd(NP-856), P=0.017, rAd(M1-89+NP-856), P=0.005, others P=0.002). All data were repeated in triplicate. Remaining 10 mice of each group were monitored 21 days for weight loss (d) and survival rates (e). P-value was 0.006 for rAd(NP-856) and <0.0001 for other Ads in survival experiment (e). Mean weight+s.d. was determined at each time point. One mouse survived in control group whose weight loss was not included. One-way ANOVA was applied to compare virus titers and pathology grade. χ2-test was performed to compare the survival rates.
