Figure 6 | Gene Therapy

Figure 6

From: Adenovirus-mediated artificial MicroRNAs targeting matrix or nucleoprotein genes protect mice against lethal influenza virus challenge

Figure 6

Influenza targeting Ads protected ICR mice partially from lethal challenge of H5N1. ICR mice (n=15) vaccinated with indicated Ads were challenged 24 h later with 5LD50 of H5N1. Five mice from each group were euthanized and dissected 5 d.p.i. Left upper lung lobes were stained with hematoxylin and eosin (a) and scored for histological changes (b) (P<0.0001). Lung viral RNA copies were determined by absolute quantification RT-PCR (c). P-value was 0.027 for both Ads. All data were repeated in triplicate. Remaining 10 mice of each group were monitored 21 days for weight loss (d) and survival rates (e) (survival experiment, rAd(M1-89+M2-117), P=0.085, rAd(NP-969), P=0.035). One-way ANOVA was applied to compare viral copies and pathology grade. χ2-square test was performed to compare the survival rates.

Back to article page