Figure 3 | Gene Therapy

Figure 3

From: Safety and antitumor effect of oncolytic and helper-dependent adenoviruses expressing interleukin-12 variants in a hamster pancreatic cancer model

Figure 3

scIL-12-TM retains biological activity. HEK-293 cells were transfected with pC-scIL-12-miR, pC-scIL-12-TM or empty plasmids (control). (a) Twenty-four hours later, transfected cells were incubated with murine splenocytes for 1 h. Splenocytes were then separated from HEK-293 cells, washed and maintained for additional 48 h until mRNA was extracted to analyze expression of IFNγ by qRT-PCR. (b) scIL-12 concentration was determined in conditioned media from transfected cells, and the indicated amounts (horizontal axis) were used to stimulate murine splenocytes. Expression of murine IFNγ was analyzed by qRT-PCR 48 h later. (c) Mouse or hamster splenocytes were stimulated with supernatants containing 10 ng ml–1 of scIL-12. In all cases, values correspond to the relative IFNγ mRNA content, using β-actin as an internal reference and applying the formula 2(Ct βactin-IFNγ)x10 000. **P<0.01.

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