Figure 4 | Gene Therapy

Figure 4

From: Safety and antitumor effect of oncolytic and helper-dependent adenoviruses expressing interleukin-12 variants in a hamster pancreatic cancer model

Figure 4

Membrane anchoring of scIL-12 in cells infected with OAVs. HaP-T1 cells were infected with the indicated OAVs at MOI 2. Supernatants and cells were collected separately 24 h later. (a) Concentration of scIL-12 in conditioned media. (b) Percentage of cells displaying scIL-12 in their surface, determined by flow cytometry. (c) HaP-T1 cells were infected with OAV-scIL-12-miR (squares) or OAV-scIL-12-TM (inverted triangles) at the indicated MOIs, and the concentration of IL-12 in the conditioned media was determined 24 h later. **P<0.01, ***P<0.001.

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