Figure 6
Effect of RASSF1A expression on the regulation of HIF-1α, caspase 3, MMP-2 and cyclinD1 protein expression in SGC7901 cells under normoxic (N) or hypoxic (H) conditions. (a) Western blot analysis of HIF-1α, caspase 3, MMP-2, cyclinD1 and β-actin in SGC7901, SGC7901/NC and SGC7901/5HC-RASSF1A cells. β-Actin was used as the internal control. (b) Summarized data of MMP-2 protein in cells. **P<0.01 vs SGC7901 or SGC7901/NC cells under hypoxic conditions and SGC7901/5HC-RASSF1A cells under normoxic conditions; (c) Summarized data of caspase 3 protein in cells. **P<0.01 vs SGC7901 or SGC7901/NC cells under hypoxic conditions; (d) Quantification of cyclinD1 protein expression in cells, **P<0.01 vs SGC7901/5HC-RASSF1A cells under normoxic conditions.
