Figure 10 | Gene Therapy

Figure 10

From: A new plasmid-based microRNA inhibitor system that inhibits microRNA families in transgenic mice and cells: a potential new therapeutic reagent

Figure 10

PMIS-miR-17-18-19-92 function in mice. (a) RT-PCR of endogenous mature miR-17 and miR-18a in PMIS-miR-17-18 transgenic mice and control littermates. Taqman probes were used to analyze miR expression from mouse tails. (b) RT-PCR of endogenous mature miR-19a and miR-92a in PMIS-miR-19-92 transgenic mice and control littermates. Taqman probes were used to analyze miR expression from mouse tails. (c) PMIS-miR-17-18 and PMIS-miR-19-92 mice were mated to produce the PMIS-miR-17-18-19-92 embryos that are perinatally lethal. The E18.5 PMIS-miR-17-18-19-92 embryos are smaller than WT embryos. (d) The weight of the PMIS-miR-17-18-19-92 embryos are less than their littermates. (e) The PMIS-miR-17-18-19-92 embryos have small lungs. (f) qPCR of PMIS-miR-17-18 and PMIS-miR-200b levels in transgenic mice expressing these constructs. Total RNA was isolated from E18.5 mandible tissue.

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