Figure 12 | Gene Therapy

Figure 12

From: A new plasmid-based microRNA inhibitor system that inhibits microRNA families in transgenic mice and cells: a potential new therapeutic reagent

Figure 12

Craniofacial and bone defects in PMIS-miR-17-18, PMIS-miR-19-92 and PMIS-miR-17-18-19-92 embryos. E18.5 embryos were harvested and heads were stained with Alcian blue and Alizarin red to detect cartilage and bone, respectively. Cross-lines were set to WT size limits and copied onto mutant heads. PMIS-miR-17-18 embryos have a reduced cranial width compared with WT but normal ossification. PMIS-miR-19-92 embryos show reduced width and anterior–posterior length but normal ossification. However, PMIS-miR-17-18-19-92 embryos have reduced anterior–posterior length, reduced cranial width and a delay in ossification of the interparietal (IP) bone. A cleft palate was identified in PMIS-miR-17-18-19-92 E18.5 embryos, coronal sections, hematoxylin and eosin (H&E) staining (bottom panels, arrows).

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