Figure 2 | Gene Therapy

Figure 2

From: A new plasmid-based microRNA inhibitor system that inhibits microRNA families in transgenic mice and cells: a potential new therapeutic reagent

Figure 2

Efficiency of the PMIS. (a) PMIS-miR inhibitors reduce specific miR expression. RT-PCR of mature endogenous miR-17 and miR-200c from 293 cells transfected with the indicated PMIS inhibitor. (b) Northern blot of mature endogenous miRs and the PMIS construct from 293 cells transfected with PMIS vector or PMIS-miR-17-18. U6 RNA is shown as a loading control. (c) Western blot of Bim, a known target of miR-17 from cells transfected with the PMIS vector, PMIS-miR-17 or PMIS-miR-200a. In addition, a western blot of PTEN, identified as a target of miR-17. β-Tubulin and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) are shown as loading controls.

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