Figure 4 | Gene Therapy

Figure 4

From: A new plasmid-based microRNA inhibitor system that inhibits microRNA families in transgenic mice and cells: a potential new therapeutic reagent

Figure 4

Knockdown of the miR-200 family with a single plasmid. (a) Sequence of mature miR-200 family members. The underlined nucleotide indicates the different ribonucleotide between two subfamilies in the seed region (bold). (b) Inhibition of miR-200a-3p with different PMIS-miR inhibitors. PMIS empty vector or various PMIS-miR inhibitors were co-transfected with miR-200a expression plasmid and miR reporter vector containing a miR-200a binding site into HEK 293FT cell. Rluc and Fluc activity was measured 48 h after transfection. (c) Inhibition of miR-141-3p with different PMIS-miR inhibitors. (d) Inhibition of miR-200b-3p with different PMIS-miR inhibitors. (e) Inhibition of miR-200c-3p with different PMIS-miR inhibitors. (f) Inhibition of miR-429-3p with different PMIS-miR inhibitors. (g) A single plasmid expressing both PMIS-miR-200a-200b inhibits all five members of the miR-200 family. The miR-17 reporter was used as a control. T-test was performed against the sample, marked by arrow. *P<0.05.

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