Figure 5 | Gene Therapy

Figure 5

From: A new plasmid-based microRNA inhibitor system that inhibits microRNA families in transgenic mice and cells: a potential new therapeutic reagent

Figure 5

Efficiency of PMIS-miR inhibitor targeting noncanonical miRs. (a) Efficiency of PMIS-miR inhibitors targeting intronic miRs that bypass Drosha processing miR-877-5p and miR-1224-5p, Dicer-independent miR-451a and in vitro transcribed siRNA. PMIS-miR inhibitor targeting Rluc mRNA served as a control. Rluc and Fluc were assayed as in previous figures. (b) As an additional control, PMIS-anti-Rluc had no effect on luciferase expression and activity. (c) The BMPR2 3′UTR was cloned into the luciferase vector and co-transfected with PMIS-miR-17-18, vector only or pSil-miR-17 (overexpression) in 293 cells.

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