Figure 7

PMIS-miR inhibitors are relatively stable in the cell. Cells stably expressing PMIS-miR-200b were seeded at identical concentrations into six- well plates and subjected to actinomycin D treatment for 7 days. After 1, 3, 5 and 7 days, cells were harvested, total RNA harvested and PMIS-miR-200b was analyzed by RT-PCR (top panel). β-Actin was used as a control. The bottom panel shows the 28 S, 18 S and 5 S RNAs as controls. Another control contained the isolated total RNA without reverse transcription (no RT control) used in the PCR reaction to detect possible PMIS DNA; however, no PMIS DNA was detected in the samples.