Figure 8
Genome-wide analyses of PMIS-miR-17 function. (a) Experiment design for the whole-genome-wide analysis of mRNA change after transduction with PMIS-miR-17. (b and c) Changes in abundance of mRNAs in PMIS-miR-17-expressing 293 cells were monitored by microarrays. TargetScan was used to predict miR-17 targets. We further separate miR-17 targets into two groups, one with context+ score <−0.30 and the other with context+ score >−0.30. Distributions of changes (0.1 unit bins) for mRNA UTRs containing no site in black line, site with score >−0.30 in red line and site with score <−0.30 in blue line. Upregulation of mRNAs with context+ score <−0.30 was significantly more than that from mRNAs with no site (P<10−13, one-sided K–S test). Comparisons between mRNAs with context+ score <−0.30 versus context+ score >−0.30, context+ score >−0.30 versus no site were also significant (P<10−8, P<10−3, respectively). (d) The 293 cells were transduced with PMIS-miR-17, total RNA harvested and randomly selected miR expression was analyzed by RT-PCR using Taqman probes (N=3).
