Figure 2
From: Ultrasound-targeted hepatic delivery of factor IX in hemophiliac mice
Co-delivery of liver-specific hFIX and reporter vectors to the mutant mouse liver. (a, b) Expression from pZY53-luc (co-delivered with pZY53-hFIX) 2 (a) and 5 (b) days after UTMD. Luciferase expression (luc) is measured as the average radiance in units of photons per s per cm2 per steradian. (c–e) hFIX expression in the mutant mouse liver by immunofluorescence. Left liver lobe immunofluorescence from treated mutant mouse 5 days after UTMD-mediated delivery of pZY53-hFIX. Hepatocytes expressing the hFIX transgene appear green and were incubated with a goat anti-human FIX primary antibody and then stained with an Alexa Fluor 488 fluorescent-conjugated secondary antibody, and nuclei are stained blue with DAPI, × 40 magnification (c, d). White arrows indicate hFIX transgene expression surrounding hepatic blood vessels. Negative control (e), liver section stained only with the secondary antibody and DAPI. (f–h) H&E staining of mutant mouse liver 5 days after UTMD-mediated delivery of hFIX (pZY53-hFIX). Left liver lobe sections, scale=50 μm (f, g) and 100 μm (h).
