Table 2 Statistical analysis for effect of pulse number on transformation of HEK293 cells using electrode array-based plasmid DNA electrotransfer

	t-value	Unadjusted P-value	Critical level	Significance
40 V Comparisons
40 V: 10 pulses vs 1 pulse	119.000	<0.001	0.003	Yes
40 V: 5 pulses vs 1 pulse	96.222	<0.001	0.004	Yes
40 V: 20 pulses vs 1 pulse	104.00	<0.001	0.004	Yes
40 V: 10 pulses vs 40 pulses	93.333	<0.001	0.004	Yes
40 V: 10 pulses vs 3 pulses	81.667	<0.001	0.005	Yes
40 V: 20 pulses vs 40 pulses	78.333	<0.001	0.005	Yes
40 V: 5 pulses vs 40 pulses	70.556	<0.001	0.006	Yes
40 V: 20 pulses vs 3 pulses	66.667	0.003	0.006	Yes
40 V: 5 pulses vs 3 pulses	58.889	0.004	0.007	Yes
40 V: 3 pulses vs 1 pulse	37.333	0.079	0.009	No
40 V: 40 pulses vs 1 pulse	25.667	0.221	0.010	No
40 V: 10 pulses vs 5 pulses	22.778	0.234	0.013	No
40 V: 10 pulses vs 20 pulses	15.000	0.471	0.017	No
40 V: 3 pulses vs 40 pulses	11.667	0.575	0.025	No
40 V: 20 pulses vs 5 pulses	7.778	0.682	0.050	No
20 V Comparisons
20 V: 10 pulses vs 1 pulse	3.642	<0.001	0.003	Yes
20 V: 10 pulses vs 40 pulses	3.393	0.001	0.004	Yes
20 V: 5 pulses vs 1 pulse	2.711	0.009	0.004	No
20 V: 5 pulses vs 40 pulses	2.461	0.017	0.004	No
20 V: 10 pulses vs 3 pulses	1.913	0.060	0.005	No
20 V: 10 pulses vs 20 pulses	1.879	0.065	0.005	No
20 V: 20 pulses vs 1 pulse	1.763	0.083	0.006	No
20 V: 3 pulses vs 1 pulse	1.730	0.089	0.006	No
20 V: 20 pulses vs 40 pulses	1.513	0.135	0.007	No
20 V: 3 pulses vs 40 pulses	1.480	0.144	0.009	No
20 V: 5 pulses vs 3 pulses	0.981	0.330	0.010	No
20 V: 5 pulses vs 20 pulses	0.948	0.347	0.013	No
20 V: 10 pulses vs 5 pulses	0.931	0.355	0.017	No
20 V: 40 pulses vs 1 pulse	0.249	0.804	0.025	No
20 V: 20 pulses vs 3 pulses	0.033	0.974	0.050	No

Abbreviation: ANOVA, analysis of variance. Statistical analysis by one-way ranked ANOVA with Holm–Sidak multiple pairwise post hoc comparisons. 20 V (n=6 per group) and 40 V (n=6 per group (except 5 pulses n=9 per group)). 40 ms duration pulses. Normality test passed (P=0.553) and equal variance test passed (P=0.775). Power of performed test with alpha 0.05:1 for both tests.

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