Figure 1

HPV DNA vaccine combined with IL2 intravaginal injection enhances the vaginal immune response. On day 0, 20 μg of DNA constructs (pcDNA3-CRT/E7 and/or pcDNA3-IL2) were submucosally injected into the vagina followed by electroporation. Mice were boosted with the same regimen on day 7. (a) Representative flow cytometric analysis for the amount of E7-specific CD8+ T cells in splenocytes. (b) Bar graph summary of the flow cytometric analysis. (c) Representative flow cytometric analysis for the amount of E7-specific CD8+ T cells in the cervicovaginal tracts of mice. (d) Bar graph summary of the flow cytometric analysis. *P<0.05; **P<0.01; NS=not significant.