Figure 4
From: Optimization of lentiviral vector production for scale-up in fixed-bed bioreactor
Total yields in the bioreactor and control flasks in 24 -, 48- and 72-h time points PT. (a, b) Total vp and TU, respectively, in bioreactors, mean±s.d. Bioreactor of run 10 was not sampled. (c, d) Total vp and TU, respectively, in controls, mean±s.d. CaPho precipitation with regular plasmid amount (340 ng cm–2), IMDM as medium; CaPho 1/2=CaPho transfection with smaller plasmid amount (170 ng cm–2), IMDM as medium; IMDM=PEI transfection, IMDM medium; high DMEM=PEI transfection, high glucose DMEM as medium; low DMEM=PEI transfection, low glucose DMEM as a medium; IMDM 400 ng=PEI transfection with higher plasmid amount (400 ng); IMDM as medium. PT, post-transfection; TU, transducing units; vp, viral particles.
