Summary
Single copy DNA sequence homologies were measured by interspecific molecular hybridization and thermal denaturation techniques for eight species of the genus Atriplex and spinach (Spinacia oleracea). Thermal stability profiles for Atriplex interspecific hybrids indicated more base pair mismatch than has been observed in most previously reported intrageneric comparisons of animal DNA. On the assumption that sequence divergence by base substitution is proportional to the time which has elapsed subsequent to speciation, the data are interpreted as indicating that lines leading to many modern Atriplex species probably originated during a single period of rapid speciation. This group includes C3 and C4 photosynthetic types from both subgenera. Thus the molecular data do not support the classical subgeneric distinction, and it is no longer necessary to postulate a polyphyletic origin for C4 photosynthesis within Atriplex.
Similar content being viewed by others
Article PDF
References
Angerer, R C, Davidson, E H, and Britten, R J. 1976. Single copy DNA and structural gene sequence relationships among four sea urchin species. Chromosoma, 56, 213–226.
Belford, H S. 1979. Single copy DNA sequence comparisons within the genus Atriplex. Ph.D thesis, University of Massachusetts (Amherst).
Belford, H S, and Thompson, W F. 1980. Single copy DNA homologies in Atriplex. I. Cross reactivity estimates and the role of deletions in genome evolution.
Björkkman, O, Troughton, J, and Nobs, M A. 1973. Photosynthesis in relation to leaf structure. Brookhaven Symp in Biology, 25, 206–226.
Britten, R J, Graham, D E, and Neufeld, B R. 1974. Analysis of repeating DNA sequences by reassociation. Methods in Enzymology, 29, 363–418.
Eden, F C, Hendrick, J P, and Gottlieb, S S. 1978. Homology of single copy and repeated sequences in chicken, duck, Japanese quail and ostrich DNA. Biochemistry, 17, 5113–5121.
Fitch, W M. 1976. Molecular evolutionary clocks. In Molecular Evolution, ed. F. J. Ayala, pp. 160–178. Sinauer Assoc., Inc. Sunderland, Mass.
Fitch, W M, and Langley, C H. 1976. Protein evolution and the molecular clock. Fed Proc, 35, 2092–2097.
Galau, G A, Chamberline, M E, Hough, B R, Britten, R J, and Davidson, E H. 1976. Evolution of repetitive and nonrepetitive DNA. In. Molecular Evolution, ed. F. J. Ayala, pp. 220–224. Sinauer Assoc., Inc. Sunderland, Mass.
Hall, H M, and Clements, F E. 1923. The genus Atriplex. In The North American Species of Artemisia, Crysothamnus, and Atriplex Carnegie Inst, of Wash Publ 326, pp. 235–342. Judd and Detweiler, Inc., Washington, D.C.
Harpold, M M, and Craig, S P. 1978. The evolution of nonrepetitive DNA in sea urchins. Differentiation, 10, 7–11.
Heiser, C B. 1973. Introgression re-examined. Bot Rev, 39, 347–366.
Kamalay, J C, and Goldberg, R B. 1980. Regulation of structural gene expression in tobacco. Cell, 19, 935–946.
Kiper, M. 1979. Gene numbers as measured by single-copy DNA saturation with raRNA are routinely overestimates. Nature, 278, 279–280.
Kohne, D E. 1970. Evolution of higher organism DNA. Quart Rev Biophysics, 33, 327–375.
Laird, C, McConaughy, B G, and McCarthy, B J. 1969. Rate of fixation of nucleotide substitution in evolution. Nature, 224, 149–154.
Martinson, H G, and Wagenaar, E G. 1977. Thermal elution chromatography of nucleic acids on hydroxyapatite. Analytical Biochem, 61, 144–154.
Mizuno, S, and MacGregor, H C. 1974. Chromosomes, DNA sequences, and evolution in salamanders of the genus Plethodon. Chromosoma, 48, 239–296.
Nambudiri, E M, Tidwell, W D, Smith, B N, and Hebbert, N P. 1978. A C4 plant from the Pliocene. Nature, 276, 816–817.
Narayan, R K J, and Rees, H. 1977. Nuclear DNA divergence among Lathyrus species. Chromosoma, 63, 101–107.
Nobs, M A. 1976. Hybridization in Atriplex. Carnegie Inst Wash Yearbook, 75, 421–423.
Osmond, C B, Björkman, O, and Anderson, D J. 1980. Physiological processes in plant ecology: Towards a synthesis with Atriplex. In. Ecological Studies, Volume 36. Springer-Verlag, New York.
Rice, N. 1972. Changes in repeated DNA in evolution. Brookhaven Symp Biol 23, 44–78.
Rosbash, M, Campo, M A, and Gummerson, K S. 1975. Conservation of cytoplasmic poly(A)-containing RNA in mouse and rat. Nature, 258, 682–686.
Shields, G F, and Straus, N A. 1975. DNA-DNA hybridization studies of birds. Evolution, 29, 159–166.
Sohn, U-I, Rothfels, K H, and Straus, N A. 1975. DNA:DNA hybridization studies in black flies. J Mol Evol 5, 75–85.
Stein, D B, Thompson, W F, and Belford, H S. 1979. Studies on DNA sequences in the Osmundaceae. J Mol Evol, 13, 215–232.
Thomasson, J R. 1978. Epidermal patterns of the lemma in some fossil and living grasses and their phylogenetic significance. Science, 199, 975–977.
Ullman, J S, and McCarthy, B J. 1973. The relationship between mismatched base pairs and thermal stability of DNA duplexes. II. Effects of deamination of cytosine. Biochem Biophys Acta, 294, 416–424.
Wilson, A C, Carlson, S S, and White, T J. 1977. Biochemical evolution Ann Rev Biochem, 46, 573–639.
Author information
Authors and Affiliations
Additional information
This research was supported in part by NSF grant DEB76-83405 and funds from the Carnegie Institution of Washington. This paper is Publication No. 717 from the Department of Plant Biology, and is based on a portion of a dissertation submitted by H.S.B. in partial fulfilment of the Ph.D requirements in the Department of Botany, University of Massachusetts, Amherst.
Rights and permissions
About this article
Cite this article
Belford, H., Thompson, W. Single copy DNA homologies in Atriplex. II. Hybrid thermal stabilities and molecular phylogeny. Heredity 46, 109–122 (1981). https://doi.org/10.1038/hdy.1981.10
Received:
Issue date:
DOI: https://doi.org/10.1038/hdy.1981.10
This article is cited by
-
The progress of DNA analyzing techniques and its impact on plant molecular systematics
Journal of Plant Research (1993)
-
Indexes to the reassociation and stability of solution DNA hybrids
Journal of Molecular Evolution (1989)
-
Non-equivalency of genera inAngiospermae: Evidence from DNA hybridization studies
Plant Systematics and Evolution (1988)
-
The emergence of a new chlorophytan system, and Dr. Kornmann's contribution thereto
Helgoländer Meeresuntersuchungen (1988)
-
Tobacco single-copy DNA is highly homologous to sequences present in the genomes of its diploid progenitors
Molecular and General Genetics MGG (1985)
