Summary
M1 plants derived from crosses using pollen irradiated with 20 Krad of γ-rays were selfed and backcrossed reciprocally to N rustica, V27. A cytogenetical examination of these progenies revealed the persistence of considerable aneuploidy and structural aberrations. It proved possible to detect several aberrant chromosomes in the M1 parents and observe their transmission rates to the offspring. Analysis indicated some post-meiotic selection against these damaged, paternal chromosomes. The intensity of this selection, however, is insufficient to account for the maternal trend previously reported for the M2 generation in N. rustica. The preferred interpretation is that the mutational damage may lead to a loss in vigour during the M2 and equivalent generations.
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Werner, C., Cornish, M. Gene transfer in Nicotiana rustica by means of irradiated pollen. III. Cytogenetical consequences in the second generation. Heredity 53, 545–551 (1984). https://doi.org/10.1038/hdy.1984.114
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DOI: https://doi.org/10.1038/hdy.1984.114
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