Abstract
Doubled haploid systems offer the quickest method of advancing heterozygous breeding lines to homozygosity. Currently the Hordeum bulbosum technique is the most efficient method of haploid production in barley. However, at present, the efficiency of this system is reduced by the suggested need for a multiplication phase following colchicine treatment of haploid plants prior to agronomic assessment. In this paper it is demonstrated that the rankings of the H1 (the first selfed generation after colchicine treatment) and H2 (second generation) generations remain unaltered. Furthermore it is shown that the correspondence between parents and progeny or H1 and H2 is determined by the relative magnitude of the additive genetic variation to the total phenotypic variation. The production of variation following a tissue culture phase (somaclonal variation) has been reported in barley. In this study the H. bulbosum doubled haploid system did not show any evidence of generating such variation.
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Powell, W., Caligari, P., Jinks, J. et al. The use of doubled haploids in barley breeding. I. Comparison of H1 and H2 generations. Heredity 54, 261–266 (1985). https://doi.org/10.1038/hdy.1985.35
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DOI: https://doi.org/10.1038/hdy.1985.35
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