Abstract
In order to produce a double mutant, sexual crosses between a streptomycin resistant mutant of Nicotiana tabacum (SR1) and a chlorophyll-deficient mutant of N. tabacum (Albino, A1) were carried out. From their offspring of this cross, a double mutant (SA) was selected and characterised with respect to streptomycin resistance and the ability to synthesize chlorophyll. Calli initiated from SA showed resistance to streptomycin and the chlorophyll content in SA leaves was lower than that of the normal green plants. Therefore, it is expected that the double mutant established here will be useful in studies of somatic hybridisation. Somatic hybrids between any other normal plants and the SA can be selected effectively by the appearance of green plants on a selective medium containing streptomycin.
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Arnon, D I. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol, 24, 1–15.
Clausen, R E, and Cameron, D R. 1950. Inheritance in Nicotiana tabacum. XXIII. Duplicate factors for chlorophyll production. Genetics, 35, 4–10.
Hamill, J D, Pental, D, Cocking, E C, and Muller, A J. 1983. Production of a nitrate reductase deficient streptomycin resistant mutant of Nicotiana tabacum for somatic hybridisation studies. Heredity, 50, 197–200.
Loschivo, F, Giovinazzo, G, and Terzi, M. 1983. 8-Azaguanine resistant carrot cell mutants and their use as universal hybridizers. Mol Gen Genet, 192, 326–329.
Maliga, P, Sz-Breznovits, A, and Marton, L. 1973. Strep-tomycin-resistant plants from callus culture of haploid tobacco. Nature New Biol, 244, 29–30.
Maliga, P, Sz-Breznovits, A, Marton, L, and Joo, F. 1975. Non-Mendelian streptomycin-resistant tobacco mutant with altered chloroplasts and mitochondria. Nature, 255, 401–402.
Moav, R, and Cameron, D R. 1960. I. The expression of instability in N tabacum X N. plumbaginifolia. Am J Bot, 47, 87–93.
Murashige, T, and Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant, 15, 473–497.
Nitsch, J P, and Nitsch, C. 1969. Haploid plants from pollen grains. Science, 163, 85–87.
Pental, D, Hamill, J D, and Cocking, E C. 1984. Somatic hybridization using a double mutant of Nicotiana tabacum. Heredity, 53, 79–83.
Uchimiya, H, and Murashige, T. 1974. Evaluation of parameters in the isolation of viable protoplasts from cultured tobacco cells, Plant Physiol, 54, 936–944.
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Toki, S., Kameya, T. Production of a chlorophyll deficient streptomycin resistant mutant of Nicotians tabacum for somatic hybridisation studies. Heredity 59, 223–226 (1987). https://doi.org/10.1038/hdy.1987.116
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DOI: https://doi.org/10.1038/hdy.1987.116
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