Abstract
The possible existence of chiasma terminalization in the grasshopper Chorthippus jucundus was tested in four males by means of comparisons between chiasma locations at diplotene and metaphase I within L3, M4 and M5 bivalents. Diplotene cells were stained by a C-banding technique to recognize heterochromatic regions, especially the centromeric ones, whereas metaphase I cells were stained by a silver staining technique that visualizes a core-like structure that extends through each homologous chromosome. The core is very pronounced at kinetochores and forms cross-shaped configurations at chiasmata. No evidence of chiasma terminalization has been found.
Similar content being viewed by others
Article PDF
References
Albini, S M, and Jones, G H. 1984. Synaptonemal-complex associated centromeres and recombination nodules in plant meiocytes prepared by an improved surface-spreading technique. Exp Cell Res, 155, 588–592.
Allen, J W. 1979. BrdU-Dye characterization of late replication and meiotic recombination in Armenian hamster germ cells. Chromosoma, 74, 189–207.
Bernelot-Moens, C, and Moens, P B. 1986. Recombination nodules and chiasma localization in two Orthoptera. Chromosoma, 93, 220–226.
Darlington, C D. 1929. Chromosome behaviour and structural hybridity in the Tradescantiae. J Genet, 21, 221–264.
Fox, D F. 1973. The control of chiasma distribution in the locust Schistocerca gregaria (Forskal). Chromosoma, 43, 289–328.
Hearne, E M, and Huskins, C L. 1935. Chromosome pairing in Melanoplus femur-rubrum. Cytologia, 6, 123–147.
Holm, P B, and Rasmussen, S W. 1983. Human meiosis. VII. Chiasma formation in human spermatocytes. Carlesberg Res Commun, 48, 415–156.
Hulten, M. 1974. Chiasma distribution at diakinesis in the normal human male. Hereditas, 76, 55–78.
Imai, H T, and Moriwaki, K. 1982. A re-examination of chiasma terminalization and chiasma frequency in male mice. Chromosoma, 85, 439–452.
Jones, G H. 1977. A test for early terminalization of chiasmata in diplotene spermatocytes of Schistocerca gregaria. Chromosoma, 63, 287–294.
Jones, G H. 1978. Giemsa C-banding of rye meiotic chromosomes and the nature of “terminal” chiasmata. Chromosoma, 66, 45–57.
Jones, G H. 1984. The control of chiasma distribution. In Evans, C. W. and Dickinson, H. G. (eds), Controlling Events in Meiosis. The Company of Biologists Ltd., Cambridge, pp. 293–320.
Jones, G H. 1987. Chiasmata. In Moens, P. B. (ed.), Meiosis, Academic Press, pp 213–244.
Kanda, N, and Kato, H. 1980. Analysis of crossing over in mouse meiotic cells by BrdU labelling technique. Chromosoma, 78, 113–121.
Laurie, D A, and Jones, G H. 1981. Inter-individual variation in chiasma distribution in Chorthippus brunneus (Orthoptera: Acrididae). Heredity, 47, 409–416.
Laurie, D A, Hulten, M, and Jones, G H. 1981. Chiasma frequency and distribution in a sample of human males: chromosomes 1, 2 and 9. Cytogenet Cell Genet, 31, 153–166.
Loidl, J. 1979. C-Band proximity of chiasmata and absence of terminalization in Allium flavum (Liliaceae). Chromosoma, 73, 45–51.
Loidl, J. 1987. Heterochromatin and differential chromosoma staining in meiosis. Biol Zentralbl, 106, 641–662.
Maguire, M P. 1979. Direct cytological evidence for true terminalization of chiasmata in maize. Chromosoma, 71, 283–287.
Maguire, M P. 1981. Chiasma maintenance and terminalization across a homoeologous bivalent segment. Chromosoma, 82, 107–112.
Mascarello, J T. 1980. Meiotic chromosomes of antelope squirrels. Cytogenet Cell Genet, 26, 104–107.
Maudlin, I, and Evans, E P. 1980. Chiasma distribution in mouse oocytes during diakinesis. Chromosome, 80, 49–56.
Orellana, J, and Giraldez, R. 1981. Metaphase I bound arms and crossing-over frequency in rye I. Open pollinated varieties. Chromosoma, 84, 439–449.
Polani, P E, Crolla, J A, Selles, H J, and Moir, F. 1979. Meiotic crossing-over exchange in the female mouse visualised by BrdU substitution. Nature, 278, 348–349.
Rufas, J S, Esponda, P, and Gosalvez, J. 1985. NOR and nucleolus in the spermatogenesis of Acridoid grasshoppers. Genetica, 66, 139–144.
Santos, J L, and Giraldez, R. 1978. The effect of C-hetero-chromatin on chiasma terminalization in Chorthippus bigut-tulus L. (Acrididae, Orthoptera). Chromosoma, 70, 59–66.
Santos, J L. 1980. Variacion de la heterocromatina constitutiva en el cariotipo de los Acridoidea y su efecto en el comportamiento cromosomico en meiosis. Tesis Doctoral. Universidad Complutense de Madrid.
Santos, J L, Ciprés, G, and Lacadena, J R. 1987. Metaphase I chiasmata in silver-stained cores of bivalents in grasshopper spermatocytes. Genome, 29, 235–238.
Solari, A J, and Counce, S C. 1977. Synaptonemal complex karyotyping in Melanoplus differentialis. J Cell Set, 26, 229–250.
Solari, A J. 1980. Synaptonemal complexes and associated structures in microspread human spermatocytes. Chromosoma, 81, 315–337.
Southern, D I. 1967. Chiasma distribution in Truxaline grasshoppers. Chromosoma, 22, 164–191.
Tease, C, and Jones, G H. 1978. Analysis of exchanges in differentially stained metiotic chromosomes of Locusta migratoria after BrdU-substitution and FPG staining. Chromosoma, 69, 163–178.
Von Wettstein, D, Rasmussen, S W, and Holm, P B. 1984. The synaptonemal complex in genetic segregation. Ann Rev Genet, 18, 331–413.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Santos, J., Ciprés, G. & Lacadena, J. A quantitative study of chiasma terminalization in the grasshopper Chorthippus jucundus. Heredity 62, 51–57 (1989). https://doi.org/10.1038/hdy.1989.7
Received:
Issue date:
DOI: https://doi.org/10.1038/hdy.1989.7
