Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Advertisement

Heredity
  • View all journals
  • Search
  • Log in
  • Content Explore content
  • About the journal
  • Publish with us
  • Sign up for alerts
  • RSS feed
  1. nature
  2. heredity
  3. original article
  4. article
Variation of ribosomal gene spacer length among wild and cultivated banana
Download PDF
Download PDF
  • Original Article
  • Published: 01 February 1992

Variation of ribosomal gene spacer length among wild and cultivated banana

  • C Lanaud2,
  • H Tezenas du Montcel1,
  • M P Jolivot1,
  • J C Glaszmann1 &
  • …
  • D Gonzalez de Leon1 

Heredity volume 68, pages 147–156 (1992)Cite this article

  • 1581 Accesses

  • 43 Citations

  • Metrics details

Abstract

The diversity of rDNA spacer length (IGS) of 107 wild and cultivated clones of banana belonging to different genetic groups, was studied using a sulphonated wheat rDNA probe. The length of the rDNA unit was between 10 and 12.6 kb and was highly variable. Fifteen different types of IGS could be observed. IGS diversity within the acuminata complex was greater among the diploid cultivars than among the triploid cultivars. Variation was also found among BB genotypes and confirms the diversity of this species. As opposed to previous studies, based on enzymatic or poly-phenolic markers, there was no obvious relation between the rDNA structure and the classification into genetic groups. More particularly, the IGS variations did not differentiate between the A and B genomes. This analysis, however, indicates a relationship between the geographical origin and the IGS structure of Thai and Indonesian clones.

Similar content being viewed by others

Comparative RNA-seq analysis of resistant and susceptible banana genotypes reveals molecular mechanisms in response to banana bunchy top virus (BBTV) infection

Article Open access 31 October 2023

Screening of exotic banana accessions for their resistance to fusarium wilt race 1 and tropical race 4 in India

Article Open access 11 July 2025

Insulin signaling regulates R2 retrotransposon expression to orchestrate transgenerational rDNA copy number maintenance

Article Open access 04 January 2025

Article PDF

References

  • Appels, R, and Dvorak, J. 1982. The wheat ribosomal DNA spacer region: its structure and variation in populations and among species. Theor Appl Genet, 63, 337–348.

    Article  CAS  PubMed  Google Scholar 

  • Appels, R, and Honeycutt, R L. 1986. rDNA: evolution over a billion years. In: Dutta, S. K. (ed.) DNA Systematics, Vol II: Plants CRC Press, Inc., Boca Raton, Florida, pp. 81–135.

    Google Scholar 

  • Benzecri, J P. 1973. L'analyse de données. Tome 2: L'analyse des correspondances. Dunod ed. Paris. 616 pp.

  • Champion, J. 1967. Les bananiers et leur culture. 1. Botanique et génétique. IFAC, SETCO ed. Paris, 214 pp.

  • Cirad/Irfa. Rapports annuels IRFA-Neufchâteau (Guadeloupe). 1986, 1987, 1988/1989.

  • Cluster, P D, Marinkovic, R, Allard, R W, and Ayala, F J. 1987. Correlations between development rates, enzyme activities, ribosomal DNA spacer-length phenotypes, and adaptation in Drosophila melanogaster. Proc Natl Acad Sci, USA 84, 610–614.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Coen, E S, Strachen, T, and Dover, G A. 1982. Dynamics of concerted evolution of ribosomal DNA and histone gene families in the melanogaster species subgroup of Drosophila. J Mol Biol, 158, 17–35.

    Article  CAS  PubMed  Google Scholar 

  • Cordesse, F, Second, G, and Delseny, M. 1990. Ribosomal gene spacer length variability in cultivated and wild rice species. Theor Appl Genet, 79, 81–88.

    Article  CAS  PubMed  Google Scholar 

  • Cullis, C A. 1981. Environmental induction of heritable changes in flax: defined environments inducing changes in rDNA and peroxidase isozyme band pattern. Heredity, 47, 87–94.

    Article  CAS  Google Scholar 

  • Cullis, C A. 1986. Plant DNA variation and stress. In: Gustafson, J. P., Stebbins, G. L. and Ayala, F. J. (eds) Genetics, Development and Evolution. Plenum Publishing Co., New York, pp. 143–155.

    Chapter  Google Scholar 

  • Dellaporta, S L, Woods, J, and Hicks, J B. 1983. A plant DNA preparation. Version II. Plant Mol Biol Rep, 4, 19–21.

    Article  Google Scholar 

  • Dessauw, D. 1987. Etude des facteurs de stérilité du bananier MMusa spp.) et des relations cytotaxinomiques entre M. acuminata Colla et M. balbisiana Colla. Thèse de doctorat. Université Paris Sud, Orsay. 162 pp.

  • Dodds, K S. 1943. Genetical and cytological studies of Musa. V. Certain edible diploids. J Genet, 45, 123–138.

    Article  Google Scholar 

  • Dodds, K S, and Simmonds, N W. 1946. Genetical and cytological studies of Musa. VIII. The formation of polyploid spores. J Genet, 47, 223–241.

    Article  CAS  PubMed  Google Scholar 

  • Dodds, K S, and Simmonds, N W. 1948. Genetical and cytological studies of Musa. IX. The origin of an edible diploid and the significance of interspecific hybridization in the Banana complex. J Genet, 48, 285–296.

    Article  CAS  PubMed  Google Scholar 

  • Flavell, R B, O'Dell, M, Sharp, P, Nevo, E, and Beiles, A. 1986. Variation in the intergenic spacer of ribosomal DNA of wild wheat, Triticum dicoccoides, in Israel. Mol Biol Evol, 3, 547–558.

    CAS  Google Scholar 

  • Gerlach, W L, and Bedbrook, J R. 1979. Cloning and characterization of ribosomal RNA genes from wheat and barley. Nuc Acids Res, 7, 1869–1885.

    Article  CAS  Google Scholar 

  • Glaszmann, J C. 1988. Geographic pattern of variation among Asian native cultivars (Oryza sativa L.) as evaluated with fifteen isozyme loci. Genome, 30, 782–792.

    Article  Google Scholar 

  • Glaszmann, J C, Fautret, A, Noyer, J L, Feldmann, P, and Lanaud, C. 1989. Biochemical genetic markers in sugarcane. Theor Appl Genet, 78, 537–543.

    Article  CAS  PubMed  Google Scholar 

  • Glaszmann, J C, Lu, Y H, and Lanaud, C. 1990. Variation of nuclear ribosomal DNA in sugarcane. J Genet Breed, 44, 191–198.

    Google Scholar 

  • Guadet, J, Julien, J, Lafay, J F, and Brygoo, Y. 1989. Phylogeny of some Fusarium species, as determined by large-subunit rRNA sequence comparison. Mol Biol Evol, 6, 227–242.

    CAS  PubMed  Google Scholar 

  • Hemleben, V, Ganal, M, Gerstner, J, Schiebel, K, and Torres, R A. 1988. Organization and length heterogeneity of plant ribosomal RNA genes. In: Kahl, G. (ed.) Architecture of Eukaryotic Genes. Verlagsgesellschaft mbH. Weinheim pp. 371–383.

    Google Scholar 

  • Horry, J P. 1989. Chimiotaxonomie et organisation génétique dans le genre Musa. Thèse de Doctorat, Université Paris XI, 105 pp.

  • Horry, J P, and Jay, M. 1988. Distribution of anthocyanins in wild and cultivated banana varieties. Phytochemistry, 27, 2667–2672.

    Article  CAS  Google Scholar 

  • Hutchinson, D J. 1966. Translocation configurations in a diploid banana. Can. J Genet Cytol, 8, 184–187.

    Article  Google Scholar 

  • Jarret, R L. 1987. Biochemical/genetic markers and their uses in the Genus Musa. In: Persley, G. J. and De Langhe, E. (eds) Banana and Plantain Breeding Strategies, ACIAR Proceedings no. 21, Cairns, Australia pp. 182–186.

    Google Scholar 

  • Jarret, R L, and Litz, R E. 1986a. Enzyme polymorphism in Musa acuminata. Coll. J Hered, 77, 183–186.

    Article  Google Scholar 

  • Jarret, R L, and Litz, R E. 1986b. Isozymes as genetic markers in bananas and plantains. Euphytica, 35, 539–549.

    Article  CAS  Google Scholar 

  • Lebacq, P, Squallis, D, Duchenne, M, Pouletty, P, and Joannes, M. 1988. A new sensitive non isotopic method using sulfonated probes to detect picogram quantities of specific DNA sequences on blot hybridization. J Biochem Biophys Meth 15, 255–266.

    Article  CAS  PubMed  Google Scholar 

  • Moss, T. 1983. A transcriptional function for the repetitive ribosomal spacer in Xenopus laevis. Nature, 302, 223–230.

    Article  CAS  PubMed  Google Scholar 

  • McMullen, M D, Hunter, B, Phillips, R L, and Rubenstein, I. 1986. The structure of the maize ribosomal DNA spacer region. Nuc Acids Res, 14, 4953–4967.

    Article  CAS  Google Scholar 

  • Reeder, R H, Roan, J G, and Dunaway, M. 1983. Spacer regulation of Xenopus ribosomal gene transcription: competition in oocytes. Cell, 35, 449–456.

    Article  CAS  PubMed  Google Scholar 

  • Rocheford, T R, Osterman, J C, and Gardner, C O. 1990. Variation in the ribosomal DNA intergenic spacer of a maize population mass-selected for high grain yield. Theor Appl Genet, 79, 793–800.

    Article  CAS  PubMed  Google Scholar 

  • Rogers, S O, and Bendich, A J. 1987. Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. Plant Mol Biol, 9, 509–520.

    Article  CAS  PubMed  Google Scholar 

  • Rogers, S O, Honda, S, and Bendich, A J. 1986. Variation in the ribosomal RNA genes among individuals of Vicia faba. Plant Mol Biol, 6, 339–345.

    Article  CAS  PubMed  Google Scholar 

  • Saghai Maroof, M A, Allard, R W, and Zhang, Q. 1990. Genetic diversity and ecogeographical differentiation among ribosomal DNA alleles in wild and cultivated barley. Proc Natl Acad Sci, USA, 87, 8486–8490.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Saghai Maroof, M A, Soliman, K M, Jorgensen, R A, and Allard, R W. 1984. Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci, USA, 81, 8014–8018.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sano, Y, and Sano, R. 1989. Variation of the intergenic spacer region of ribosomal DNA in cultivated and wild rice species. Genome, 33, 209–218.

    Article  Google Scholar 

  • Shepherd, K. 1987. Translocations in Musa acuminata. In: Genetic improvement of Bananas and Plantains, and the INIBAP Project on International Musa Germplasm exchanges. INIBAP 16–18 mars. 1987. Bogota. Colombie.

  • Simmonds, N W. 1962. The Evolution of the Bananas. Longmans London.

    Google Scholar 

  • Simmonds, N W. 1966. Bananas. Longmans, London.

    Google Scholar 

  • Simmonds, N W, and Shepherd, K. 1955. The taxonomy and origins of the cultivated bananas. J Linn Soc (Bot), 55, 302–312.

    Article  Google Scholar 

  • Tezenas Du Montcel, H. 1985. Evaluation des collections d'Asie du Sud-Est (Thaīlande, Malaisie, Indonésie). CIRAD/IRFA. Rapport de mission 30/3–24/4.

  • Tezenas Du Montcel, H. 1989. L'importance de la taxonomie pour l'amélioration génétique des bananiers. ACORBAT 89. IX Reunion 25–30/09. Merida Venézuela. Proceedings (in press).

  • Toloczyki, C, and Feix, G. 1986. Occurrence of 9 homologous repeat units in the external spacer region of a nuclear maize rRNA gene unit. Nuc Acids Res, 14, 4969–4985.

    Article  CAS  Google Scholar 

  • Wang, Z Y, and Tanksley, S D. 1989. Restriction fragment length polymorphism in Oryza sativa L. Genome, 32, 1113–1118.

    Article  CAS  Google Scholar 

  • Wilson, G B. 1946. Cytological studies in the Musae. II. Meiosis in some diploid clones. Genetics, 31, 475–482.

    PubMed  PubMed Central  Google Scholar 

  • Zimmer, E A, Jupe, E R, and Walbot, V. 1988. Ribosomal gene structure, variation and inheritance in maize and its ancestors. Genetics, 120, 1125–1136.

    CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. BIOTROP, CIRAD, BP 5035, Montpellier Cédex, 34032, France

    H Tezenas du Montcel, M P Jolivot, J C Glaszmann & D Gonzalez de Leon

  2. IRFA, c/o, INIBAP, 7bd de la Lironde, Montferrier-sur-Lez, 34980, France

    C Lanaud

Authors
  1. C Lanaud
    View author publications

    Search author on:PubMed Google Scholar

  2. H Tezenas du Montcel
    View author publications

    Search author on:PubMed Google Scholar

  3. M P Jolivot
    View author publications

    Search author on:PubMed Google Scholar

  4. J C Glaszmann
    View author publications

    Search author on:PubMed Google Scholar

  5. D Gonzalez de Leon
    View author publications

    Search author on:PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lanaud, C., du Montcel, H., Jolivot, M. et al. Variation of ribosomal gene spacer length among wild and cultivated banana. Heredity 68, 147–156 (1992). https://doi.org/10.1038/hdy.1992.23

Download citation

  • Received: 26 February 1991

  • Issue date: 01 February 1992

  • DOI: https://doi.org/10.1038/hdy.1992.23

Share this article

Anyone you share the following link with will be able to read this content:

Sorry, a shareable link is not currently available for this article.

Provided by the Springer Nature SharedIt content-sharing initiative

Keywords

  • banana
  • genetic diversity
  • intergenic spacer (IGS)
  • rDNA
  • RFLP

This article is cited by

  • Phylogenetic implications of the internal transcribed spacers of nrDNA and chloroplast DNA fragments of Musa in deciphering the ambiguities related to the sectional classification of the genus

    • Animos Lamare
    • Arman Mahmoudi Otaghvari
    • Satyawada Rama Rao

    Genetic Resources and Crop Evolution (2017)

  • Phylogenetic relationships between Ensete and Musa species as revealed by the trnT trnF region of cpDNA

    • Endashaw Bekele
    • Masayoshi Shigeta

    Genetic Resources and Crop Evolution (2011)

  • Development and assessment of Diversity Arrays Technology for high-throughput DNA analyses in Musa

    • Ange-Marie Risterucci
    • Isabelle Hippolyte
    • Jean-Christophe Glaszmann

    Theoretical and Applied Genetics (2009)

  • Molecular phylogeny of banana cultivars from Thailand based on HAT-RAPD markers

    • Suttada Ruangsuttapha
    • Klaus Eimert
    • Kamnoon Kanchanapoom

    Genetic Resources and Crop Evolution (2007)

  • Diploid Ancestors of Triploid Export Banana Cultivars: Molecular Identification of 2n Restitution Gamete Donors and n Gamete Donors

    • Louis-Marie Raboin
    • Françoise Carreel
    • Pierre J.L. Lagoda

    Molecular Breeding (2005)

Download PDF

Advertisement

Explore content

  • Research articles
  • Reviews & Analysis
  • News & Comment
  • Podcasts
  • Current issue
  • Collections
  • Follow us on Twitter
  • Sign up for alerts
  • RSS feed

About the journal

  • Journal Information
  • Open access publishing
  • About the Editors
  • Contact
  • About the Partner
  • For Advertisers
  • Subscribe

Publish with us

  • For Authors & Referees
  • Language editing services
  • Open access funding
  • Submit manuscript

Search

Advanced search

Quick links

  • Explore articles by subject
  • Find a job
  • Guide to authors
  • Editorial policies

Heredity (Heredity)

ISSN 1365-2540 (online)

ISSN 0018-067X (print)

nature.com sitemap

About Nature Portfolio

  • About us
  • Press releases
  • Press office
  • Contact us

Discover content

  • Journals A-Z
  • Articles by subject
  • protocols.io
  • Nature Index

Publishing policies

  • Nature portfolio policies
  • Open access

Author & Researcher services

  • Reprints & permissions
  • Research data
  • Language editing
  • Scientific editing
  • Nature Masterclasses
  • Research Solutions

Libraries & institutions

  • Librarian service & tools
  • Librarian portal
  • Open research
  • Recommend to library

Advertising & partnerships

  • Advertising
  • Partnerships & Services
  • Media kits
  • Branded content

Professional development

  • Nature Awards
  • Nature Careers
  • Nature Conferences

Regional websites

  • Nature Africa
  • Nature China
  • Nature India
  • Nature Japan
  • Nature Middle East
  • Privacy Policy
  • Use of cookies
  • Legal notice
  • Accessibility statement
  • Terms & Conditions
  • Your US state privacy rights
Springer Nature

© 2025 Springer Nature Limited