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Secondary constrictions and NORs of of Lathyrus investigated by silver staining and in-situ hybridization
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  • Original Article
  • Published: 01 May 1992

Secondary constrictions and NORs of of Lathyrus investigated by silver staining and in-situ hybridization

  • B G Murray1,
  • M D Bennett1 &
  • K R W Hammett2 

Heredity volume 68, pages 473–478 (1992)Cite this article

  • 709 Accesses

  • 28 Citations

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Abstract

A variety of karyotypic differences were seen among six Lathyrus species. In part these differences are the result of different numbers of metacentric and acrocentric chromosomes in the complement but there were also clear differences in the number(s) and position(s) of secondary constrictions. Silver staining and in-situ hybridization were used to identify the nucleolar organizer regions (NORs). Silver staining also occurred at the centromere in most species and in addition at a number of non-centromeric sites in L. tingitanus. Some of these additional sites coincide with allocyclic regions revealed by the use of air-dried preparations. Unusual ‘extended’ centromeres were observed in these air-dried preparations of two of the species.

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Author information

Authors and Affiliations

  1. Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AB, UK

    B G Murray & M D Bennett

  2. DSIR Fruit and Trees, Private Bag, Auckland, New Zealand

    K R W Hammett

Authors
  1. B G Murray
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  2. M D Bennett
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  3. K R W Hammett
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Murray, B., Bennett, M. & Hammett, K. Secondary constrictions and NORs of of Lathyrus investigated by silver staining and in-situ hybridization. Heredity 68, 473–478 (1992). https://doi.org/10.1038/hdy.1992.68

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  • Received: 19 June 1991

  • Issue date: 01 May 1992

  • DOI: https://doi.org/10.1038/hdy.1992.68

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Keywords

  • in-situ
  • hybridization
  • karyotypes
  • Lathyrus
  • silver staining

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