Abstract
Genomic in situ hybridization (GISH) was used to study the nature of homoeologous chiasma formation in crosses between Triticum durum cv. Creso, homozygous for the ph1c mutation and Thinopyrum bessarabicum. The relative frequencies of wheat/wheat and wheat/Th. bessarabicum chiasma formation were determined. Pairing between apparently non-homologous Th. bessarabicum chromosomes was also observed. The potential of GISH as a tool for analysing homoeologous chiasma formation in wheat/alien hybrids is discussed.
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Forster, B F, Gorham, J, and Miller, T E. 1987. Salt tolerance of an amphidiploid between Triticum aestivum and Agropyron junceum. Plant Breeding, 98, 1–8.
Forster, B F, Miller, T E, and Law, C N. 1988. Salt tolerance of two wheat-Agropyron junceum disomic addition lines. Genome, 30, 559–564.
Gale, M D, and Miller, T E. 1987. The introduction of alien genetic variation in wheat. In: F.G.H. Lupton (ed.) Wheat Breeding: its scientific basis, pp. 173–210.
Giorgi, B. 1983. Origin, behaviour and utilization of a Ph1 mutant of durum wheat, Triticum turgidum (L.) var. durum. In: Sakamoto, S. (ed.) Proc 6th Int Wheat Genet Symp Kyoto, Japan, pp. 1033–1040.
Hutchinson, J, Miller, T E, and Reader, S M. 1983. C-banding at meiosis as a means of assessing chromosome affinities in the triticeae. Can J Genet Cytol, 25, 319–323.
Jouve, N, and Giorgi, B. 1986. Analysis of induced homoeologous pairing in hybrids between 6x triticale ph1 mutant and Triticum aestivum L. Can J Genet Cytol, 28, 696–700.
Laurie, D A, and Reymondie, S. 1991. High frequencies of fertilization and haploid seedling production in crosses between commercial hexaploid wheat varieties and maize. Plant Breeding, 106, 182–189.
Laurie, D A, and O'Donoughue, L S. 1993. Wheat × maize crosses for the production of wheat haploids. Agriculture and Forestry, 25, in press.
Naranjo, T, Fernandez-Rueda, P, Goicoechea, P G, Roca, A, and Giraldez, R. 1989. Homoeologous pairing and recombination between the long arms of group 1 chromosomes in wheat × rye hybrids. Genome, 32, 293–301.
Okamoto, M. 1957. Asynaptic effect of chromosome V. Wheat Inf Serv, 5, 6.
Riley, R, and Chapman, V. 1958. Genetic control of the cytologically diploid behaviour of hexaploid wheat. Nature, 182, 713–715.
Riley, R, Chapman, V, and Kimber, G. 1959. Genetic control of chromosome pairing in intergeneric hybrids with wheat. Nature, 183, 1244–1246.
Schwarzacher, T, Anamthawat-Jonsson, K, Harrison, G E, Islam, A K M R, Jia, J Z, King, I P, Leitch, A R, Miller, T E, Reader, S M, Rogers, W J, Shi, M, and Heslop-Harrison, J S. 1992. Genomic in situ hybridization to identify alien chromosome segments in wheat. Theor Appl Genet, 84, 778–786.
Sears, E R. 1977. An induced mutant with homoeologous pairing in common wheat. Can J Genet Cytol, 19, 585–593.
Sharp, P J, Kries, M, Shewry, P R, and Gale, M D. 1988. Location of β-amylase sequences in wheat and its relatives. Theor Appl Genet, 75, 286–290.
Tease, C, and Jones, G H. 1978. Analysis of exchanges in differentially stained meiotic chromosomes of Locusta migratoria after BrdU-substitution and FPG staining. 1. Crossover exchanges in monochiasmate bivalents. Chromosoma, 69, 163–178.
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King, I., Purdie, K., Orford, S. et al. Detection of homoeologous chiasma formation in Triticum durum × Thinopyrum bessarabicum hybrids using genomic in situ hybridization. Heredity 71, 369–372 (1993). https://doi.org/10.1038/hdy.1993.151
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DOI: https://doi.org/10.1038/hdy.1993.151
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