Abstract
Four morphological taxa of the beetle genus Pimelia (Coleoptera, Tenebrionidae) are known to exist on the Atlantic island of Tenerife. We have obtained DNA sequences for 61 individuals from these taxa across the island for a 200 bp long fragment of the mitochondrial COI gene. In addition, a restriction site polymorphism in the nuclear rRNA ITS-1 sequence was identified and screened in a sample of these individuals using the enzyme Kpn2I. The results were analysed using approaches which allow inferences to be made about the population genetic structure and the mitochondrial genealogy of these closely related beetles. The mtDNA haplotype distribution and the estimates of sequence divergence revealed the presence of two ancient mtDNA lineages which coincide with the disjunct volcanic evolution of the island. The ITS-1 polymorphism was found to be diagnostic of these two lineages. However, the morphological and mitochondrial phylogenies were found to be discordant. We argue that this is possibly the result of rapid morphological change, produced by selection in different habitats, which has been recently superimposed on an older mitochondrial DNA divergence.
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Ancochea, E, Fuster, J M, Ibarrolae, E, Cendrero, A, Coello, J, Hernan, F, Cantagrel, J M, and Jamond, C. 1990. Volcanic evolution of the Island of Tenerife (Canary Islands) in the light of new K-Ar data. J Volcanol Geotherm Res, 44, 231–249.
Anguita, F, and Hernan, F. 1975. A propagating fracture model versus a hot spot origin for the Canary Islands. Earth Planet Sci Lett, 27, 11–19.
Avanzati, A M, Baratti, M, and Bernini, F. 1994. Molecular and morphological differentiation between steganacarid mites (Acari: Oribatida) from the Canary Islands. Biol J Linn Soc, 52, 325–340.
Avise, J C. 1994. Molecular Markers, Natural History and Evolution. Chapman & Hall, New York.
Boyce, T M, Zwick, M E, and Aquadro, C F. 1994. Mitochondrial DNA in the bark weevils: phylogeny and evolution in the Pissodes strobi species group (Coleoptera: Curculionidae). Mol Biol Evol, 11, 183–194.
Bremer, K. 1988. The limits of amino acid sequence data in angiosperm phylogenetic reconstruction. Evolution, 42, 795–803.
Brown, R P, Thorpe, R S, and Baez, M. 1993. Patterns and causes of morphological population differentiation in the Tenerife skink, Chalcides viridanus. Biol J Linn Soc, 50, 313–328.
Cobolli Sbordoni, M, De Matthaeis, E, La Rosa, G, Mattocia, M, and Vigna Taglianti, A. 1991. Biochemical differentiation and divergence time in the canarian genus Eutrichopus (Coleoptera, Carabidae). In: Biogeographical aspects of insularity. Atti del Convegni Lincei, 85, 233–243.
Cooper, S J B, Ibrahim, K M, and Hewitt, G M. 1995. Postglacial expansion and genome subdivision in the European grasshopper, Chorthippus parallelus. Mol Ecol, 4, 49–60.
Davis, J I, and Nixon, K C. 1992. Populations, genetic variation, and the delimitation of phylogenetic species. Syst Biol, 41, 421–435.
Desalle, R, and Giddings, L V. 1986. Discordance of nuclear and mitochondrial DNA phylogenies in Hawaiian Drosophila. Proc Natl Acad Sci USA, 83, 6902–6906.
Desalle, R, Freedman, T, Prager, E M, and Wilson, A C. 1987. Tempo and mode of sequence evolution in mitochondrial DNA of Hawaiian Drosophila. J Mol Evol, 26, 157–164.
Español, F. 1961. Las Pimelia de las Islas Canarias. Anuario Estudios Atlànticos, 7, 487–498.
Hudson, R R, Boos, D D, and Kaplan, N L. 1992. A statistical test for detecting geographic subdivision. Mol Biol Evol, 9, 138–151.
Juan, C, Oromì, P, and Hewitt, G M. 1995. Mitochondrial DNA phylogeny and sequential colonization of Canary Islands by darkling beetles of the genus Pimelia (Teneb-rionidae). Proc R Soc B, 261, 173–180.
Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J Mol Evol, 16, 111–120.
Kingman, J F C. 1982. The coalescent. Stochast Proc Appl, 13, 235–248.
Kluge, A G, and Wolf, A J. 1993. Cladistics: what's in a word? Cladistics, 9, 193–200.
Lynch, M, and Crease, T J. 1990. The analysis of population survey data on DNA sequence variation. Mol Biol Evol, 7, 377–394.
Machado, A. 1976. Introduction to a faunal study of Canary Island laurisilva with special reference to the ground-beetles (Coleoptera, Caraboidea). In: Kunkel, G. (ed.) Biogeography and Ecology in the Canary Islands, pp. 347–412. W. Junk Publishers, The Hague, The Netherlands.
Moore, W S. 1995. Inferring phylogenies from mtDNA variation: mitochondrial-gene trees versus nuclear-gene trees. Evolution, 49, 718–726.
Oromí, P. 1979. Taxonomía numérica de las Pimelia (Col., Tenebrionidae) del archipiélago Canario. Boletin Asociación Española Entomología, 3, 103–118.
Oromí, P. 1982. Los Tenebriónidos de las Islas Canarias. Instituto de Estudios Canarios 50 Aniversario, 1, 265–292.
Schlötterer, C, Hauser, M-T, Von Haeseler, A, and Tautz, D. 1994. Comparative evolutionary analysis of rDNA ITS regions in Drosophila. Mol Biol Evol, 11, 513–522.
Swofford, D L. 1991. When are phylogenetic estimates from morphological data incongruent? In: Miyamoto, M. M and Cracraft, J. (eds) Phylogenetic Analysis of DNA Sequences, pp. 295–333. Oxford University Press, Oxford.
Swofford, D. 1993. PAUP: Phylogenetic Analysis Using Parsimony, version 3.1.1. Smithsonian Institution, Washington, DC.
Templeton, A R, Routman, E, and Phillips, C A. 1995. Separating population structure from population history: a cladistic analysis of the geographical distribution of mitochondrial DNA haplotypes in the Tiger salamander, Ambystoma tigrinum. Genetics, 140, 767–782.
Vogler, A P, and Desalle, R. 1993. Mitochondrial DNA evolution and the application of the phylogenetic species concept in the Cicindela dorsalis complex (Cole-optera: Cicindelidae). In: Desender, K. (ed.) Carabid Beetles: Ecology and Evolution, pp. 79–85. Kluwer, Dordrecht, The Netherlands.
Vogler, A P, and Desalle, R. 1994. Evolution of phylogenetic information content of the ITS-1 region in the Tiger beetle, Cicindela dorsalis. Mol Biol Evol, 11, 393–405.
Vrana, P, and Wheeler, W. 1992. Individual organisms as terminal entities: laying the species problem to rest. Cladistics, 8, 67–72.
White, T J, Bruns, T, Lee, S, and Taylor, J. 1990. Amplification and direct sequencing of fungal ribsomal RNA genes for phylogenetics. In: Innis, M. A., Gelfand, D. H., Sninsky, J. J and White, T. J. (eds) PCR Protocols A Guide to Methods and Application, pp. 79–85. Academic Press, San Diego, CA.
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Juan, C., Ibrahim, K., Oromi, P. et al. Mitochondrial DNA sequence variation and phylogeography of Pimelia darkling beetles on the Island of Tenerife (Canary Islands). Heredity 77, 589–598 (1996). https://doi.org/10.1038/hdy.1996.186
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DOI: https://doi.org/10.1038/hdy.1996.186
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