Table 5 Effect of sampling strategy on results of ETL mapping in random hybridization design of F2 plants (200 replicates)
Sampling strategy | Statistic | b0 | σ2 | ETL 1 | ETL 2 | ETL 3 | ETL 2 × ETL 3 | False-positive rate (%) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
a1 | d11 | d12 | Posi. | a2 | d21 | Posi. | a3 | Posi. |
| Posi. | Posi. | a | d | iaa | iad | idd | ||||
True value | 100.000 | — | 2.000 | 5.200 | −5.200 | 60.00 | 3.000 | 3.000 | 60.00 | 1.000 | 60.00 | 1.500 | 60.00 | 60.00 | ||||||
600 × 5a | Mean | 100.092 | 13.245 | 1.992 | 5.137 | −5.064 | 60.00 | 3.042 | 2.874 | 59.58 | 0.931 | 59.88 | 1.443 | 59.81 | 60.06 | 0.11 | 0.06 | 0.09 | 0.01 | 0.00 |
s.d. | 0.413 | 0.846 | 0.202 | 0.676 | 0.664 | 0.17 | 0.231 | 0.562 | 2.22 | 0.199 | 5.79 | 0.258 | 3.07 | 2.99 | ||||||
Power | 1.000 | 1.000 | 1.000 | 1.000 | 0.915 | 0.955 | 0.985 | |||||||||||||
300 × 10 | Mean | 100.211 | 6.575 | 1.970 | 5.172 | −5.080 | 59.99 | 3.052 | 2.928 | 59.60 | 0.925 | 58.71 | 1.437 | 58.78 | 59.66 | 0.06 | 0.10 | 0.09 | 0.00 | 0.00 |
s.d. | 0.454 | 0.598 | 0.224 | 0.703 | 0.705 | 0.29 | 0.237 | 0.508 | 2.42 | 0.200 | 6.81 | 0.306 | 6.24 | 2.30 | ||||||
Power | 1.000 | 1.000 | 1.000 | 1.000 | 0.730 | 0.940 | 0.920 | |||||||||||||
200 × 15 | Mean | 100.350 | 4.561 | 1.979 | 5.055 | −5.061 | 59.96 | 3.115 | 2.967 | 59.60 | 0.917 | 58.91 | 1.416 | 59.01 | 59.57 | 0.06 | 0.03 | 0.08 | 0.00 | 0.00 |
s.d. | 0.482 | 0.591 | 0.209 | 0.711 | 0.715 | 0.28 | 0.251 | 0.540 | 2.43 | 0.211 | 6.51 | 0.271 | 6.17 | 5.31 | ||||||
Power | 1.000 | 1.000 | 1.000 | 1.000 | 0.575 | 0.870 | 0.910 | |||||||||||||
150 × 20 | Mean | 100.474 | 3.775 | 1.991 | 5.073 | −5.031 | 59.98 | 3.140 | 3.099 | 59.85 | 0.967 | 58.43 | 1.390 | 59.03 | 59.32 | 0.00 | 0.04 | 0.04 | 0.00 | 0.00 |
s.d. | 0.543 | 0.645 | 0.244 | 0.807 | 0.817 | 0.18 | 0.267 | 0.563 | 1.22 | 0.239 | 7.87 | 0.301 | 8.09 | 8.03 | ||||||
Power | 1.000 | 1.000 | 1.000 | 1.000 | 0.350 | 0.760 | 0.700 | |||||||||||||
100 × 30 | Mean | 100.734 | 3.348 | 1.960 | 5.041 | −5.094 | 60.02 | 3.189 | 3.296 | 59.99 | 1.018 | 58.81 | 1.401 | 59.16 | 58.26 | 0.03 | 0.03 | 0.03 | 0.00 | 0.00 |
s.d. | 0.644 | 0.873 | 0.260 | 0.946 | 1.020 | 0.50 | 0.305 | 0.736 | 0.22 | 0.264 | 7.91 | 0.351 | 10.95 | 8.04 | ||||||
Power | 1.000 | 0.960 | 0.975 | 1.000 | 0.060 | 0.505 | 0.390 | |||||||||||||
