Table 1 Primers and probes for TaqMan real-time PCR for Gryllus and Wolbachia genes

From: Wolbachia plays no role in the one-way reproductive incompatibility between the hybridizing field crickets Gryllus firmus and G. pennsylvanicus

Species

Primer or probe

Sequence (5′–3′)

Position

Gryllus…

EF1α_F

CTGACCTCCGCAGCAACA

569–587

 

EF1α_R

TTGCCAGTGGTCGAACACA

612–630

 

EF1α probe (TET)

TGGCCAGGCATTCCCTCAGT

591–610

Wolbachia…

ftsZ_F

TGAAGAAGTGGATGAAAATGCAAA

729–752

 

ftsZ_R

GCCAGTTGCAAGAACAGAAACTC

800–822

 

ftsZ probe (6FAM)

ACTTTTGATCAGGCGATGGAGGGAAGA

769–795

  1. The nucleotide positions are those reported in GenBank (accession number DQ630925 for EF1α and U28195 for ftsZ).
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