Figure 3

Mean relative gene transcription (±1 s.e.m.) for seven genes using quantitative real-time PCR (qRT-PCR) on triploid and diploid Chinook salmon challenged with live Vibrio anguillarum over a 3-day period after injection. All values are normalized to an endogenous control (EF-1a) and calibrated to transcription values for sham-injected salmon sampled at the same time, Asterisks indicate significant differences (P<0.05) between diploid and triploid response at each sampling time. Ten fish were used for all challenge qRT-PCRs, whereas the sham-injected qRT-PCR assays included six fish.