Figure 2 | Heredity

Figure 2

From: Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement

Figure 2

Fluorescent in situ hybridization (FISH) with 18S ribosomal RNA (rRNA), 5S rRNA and histone H3 gene probes in 0B and 1B individuals of Dichotomius geminatus. Pachytene chromosomes from 0B individuals hybridized using 18S (a) and 5S rDNAs (b); double FISH with 18S (green) and 5S (red) rDNAs in metaphase I chromosomes of 0B individuals (c); partial metaphase I chromosomes hybridized with 5S (d) and H3 (e) probes; metaphase I chromosomes showing the distribution pattern of 18S (f), 5S (g) and H3 (h) in 1B individuals. The absence of hybridization signals on the B chromosome (f–h) and the heterochromatin highlighted after DAPI staining (a–h) must be noted. The arrows indicate the sex bivalents (Xyp), and arrowheads indicate the B chromosome. C=centromere. Bar=5 μm. A full color version of this figure is available at the Heredity journal online.

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