Figure 2
From: Contrasting modes for loss of pungency between cultivated and wild species of Capsicum
pun13 genotyping. (a) The pun13 mutation is a large insertion or deletion that results in a truncated second exon. Genotyping primers were designed based on this mutation that discriminated wild-type Pun1 and pun13. Primers 1 and 2 are anchored in exons, but primer 3 is located in the genomic sequence past the mutation site in pun13. (b) The primer set shown in A allows for co-dominant genotyping of populations segregating for pun13. Primer pair 1 and 3 produces a band of 1033 bp in the presence of the pun13 allele, while primer pair 1 and 2 produces a band of 586 bp in the presence of a wild-type Pun1 allele. The parental genotypes of C. frutescens BG2814-6 (P1), C. frutescens PI594141-np (P2) and their F1 hybrid used as the parent of the F2 can be distinguished. F2 segregants are shown: Pun1/Pun1 and pungent (P), Pun1/pun13 and pungent and pun13/pun13 and non-pungent (np). No bands are found in the negative control (NC). (c) Capsaicinoid accumulation was measured by enzyme-linked immunosorbent assay (ELISA) in red-ripe fruits of the plants shown in B. No capsaicinoids were observed in C. frutescens PI594141-np or pun13/pun13 F2 individuals. Pun1/Pun1 and Pun1/pun13 individuals had a wide range of capsaicinoid levels from less than 200 p.p.m. to more than 1000 p.p.m.
