Figure 3
From: Contrasting modes for loss of pungency between cultivated and wild species of Capsicum
Pun1 expression in fruit of wild type and mutant peppers. Pepper fruit were collected 20 and 40 days post anthesis (dpa) and RT-PCR was performed using primers that provided PCR amplification of both Pun1 and pun13 alleles from genomic DNA. Robust expression of Pun1 was observed from 20 days post anthesis fruit that were pungent, but no expression was observed in pun13, non-pungent fruit. Expression diminished in 40 days post anthesis pungent fruit. An internal ubiquitin conjugating enzyme (E2) control was equivalent in all samples. Neither band was produced in a negative control without complementary DNA (cDNA) (NC).
