Table 1 Source of plant material and summary statistics of the RT-domain-encoding-region (229 bp) of Ty1/copia-like elements isolated from five Helianthus species

Species	Accession ^a	Original collection location		Number of sequences			S^e	h^f	Nucleotide diversity
		State	Lat. (N), Long. (W)	Total ^b	Indels/ stop ^c	Full length ^d			Total		Sub-lineage B’
									π	s.d. (π)	π	s.d. (π)
H. annuus	PI 468607	Utah	37.239, 113.358	39	10	29	196	38	0.314	0.034	0.078	0.010
	PI 468477	Texas	35.192, 102.107	—	—	—	—	—	—	—	—	—
H. petiolaris	PI 468815	Utah	37.047, 112.530	48	10	38	191	46	0.194	0.033	0.077	0.008
	PI 435807	Texas	35.190, 102.065	—	—	—	—	—	—	—	—	—
H. anomalus	Ames 26095	Utah	39.744, 112.316	47	16	35	182	47	0.235	0.036	0.073	0.010
H. deserticola	Ames 26094	Utah	37.254, 113.343	46	21	27	192	43	0.278	0.033	0.083	0.012
H. paradoxus	PI 468802	Texas	30.883, 102.983	41	9	35	183	39	0.200	0.038	0.049	0.004

Abbreviations: PCR, polymerase chain reaction; RT, reverse transcriptase.
Nucleotide diversity (π) was calculated for all sequences within each species’ genome as well as for the sub-lineage B’ (see Figure 3). Accession PI 468477 (H. annuus) and accession PI 435807 (H. petiolaris) were used only in the quantitative PCR assays of element copy number.
^aUSDA-National Plant Germplasm System (http://www.ars-grin.gov/npgs/).
^bThe total number of sequences obtained.
^cThe number of sequences with indels or putative stop codons.
^dThe number of sequences without putative stop codons.
^eThe number of segregating sites.
^fThe number of unique sequences.

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