Figure 2

Testicular meiotic cells from Symphysodon aequifasciatus (a, d, g, j, m), S. haraldi (b, e, h, k, n) and S. discus (c, f, i, l, o) after Giemsa staining. (a–c) Pachytene. (d and e) Diplotene, showing a ring chromosomal chain (arrowhead) and 20 bivalents; the majority of the bivalents had two terminal chiasmata (arrows). (f) Diplotene with 30 bivalents, most of them exhibited two terminal chiasmata (arrow). (g and h) Diakinesis, showing a linear chromosomal chain (arrowheads) and several bivalents and univalents. (i) Diakinesis, showing the early separation of the chromosomal elements of a bivalent (arrow). (j and k) Metaphase I, revealing the zigzag orientation of chromosomes within the chain (arrowheads). (l) Metaphase I with bivalents aligned at the equatorial plate. (m–o) Metaphase II, with n=30 chromosomes (scale bar: 10 μm).