Table 1 Number of genotypes (N), number of alleles (A), mean expected heterozygosity (HE), test for Hardy–Weinberg equilibrium (HWE) chi-squared value (χ2), degrees of freedom (d.f.), P-value (P) and estimated null allele frequency (fnull) for 17 microsatellite loci genotyped in a total of 276 European bison from Białowieża Forest (Poland)

From: Effectiveness of microsatellite and SNP markers for parentage and identity analysis in species with low genetic diversity: the case of European bison

Locus

N

A

HE

HWE

f null

    

χ2

d.f.

P

 

AGLA232

246

2

0.008

Not done

−0.0002

  

BBJ24

270

2

0.029

Not done

−0.0019

  

BM723

245

3

0.293

0.8651

1

0.3523

+0.0373

BM757

271

4

0.508

3.5551

1

0.0594

−0.0557

BM1225

266

3

0.654

7.7257

3

0.0520

+0.0527

BM1824

270

3

0.365

0.0100

1

0.9203

+0.0042

BM4440

268

2

0.157

1.3424

1

0.2466

−0.0373

BM5004

274

2

0.097

Not done

−0.0170

  

RT29

272

3

0.168

0.0481

1

0.8264

+0.0159

TGLA57

269

3

0.176

0.0045

1

0.9467

−0.0105

TGLA126

270

3

0.460

5.1464

3

0.1614

+0.0032

TGLA261

230

4

0.167

2.0709

1

0.1501

+0.0613

Mean (12 loci)

263

2.83

0.257

    

BM2830

271

4

0.604

17.2005

3

0.0006

+0.0164

BMC1222

248

3

0.114

Not done

+0.1221

  

BOVFSH

198

5

0.507

11.0475

1

0.0009

+0.1308

ETH152

272

3

0.466

58.3139

3

<0.0001

+0.0730

TGLA53

247

3

0.478

8.4218

1

0.0037

+0.1010

Mean (17 loci)

258

3.05

0.309

    
  1. The first twelve loci are classed as well-behaved loci, whereas the remaining five show significant deviations from HWE and/or a high null allele frequency. Tests for HWE were not done if rare alleles could not be combined in such a way that the minimum expected genotype frequency in the χ2-test exceeded 1.
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