Figure 3

Quantitative trait loci (QTL) mapping of all six cuticular hydrocarbons (CHCs). Composite interval mapping (CIM) was carried out with a walking speed of 2 cM, a window size of 10 cM and forward/backwards regression on all significant background markers (see Table 1 for the number of significant markers for each CHC). The locations of the marker loci are indicated on the x axis in the conventional left to right order for Drosophila. The experiment-wide significance level of P<0.05 obtained by 1000 permutations of the trait data among marker classes is shown with a horizontal line. Only the highest significance level, for 7,11-heptacosadiene (HD) is shown. Values for the significance levels are given in Table 1. Candidate loci, which are new markers in this study, are in pink. Across the top of the figure are the boundaries of the 2-LOD intervals for each QTL.