Figure 2
From: Sex in unisexual salamanders: discovery of a new sperm donor with ancient affinities

Fluorescence genomic in situ hybridization (GISH) applied to a chromosome squash preparation of a tetraploid Ambystoma laterale –2 jefffersonianum – barbouri (LJJB) larva from Mitchell Memorial Forest (MMF). A. laterale chromosomes (14) are green, A. jeffersonianum chromosomes (28) are blue and A. barbouri chromosomes (14) are pink. The sphere at the bottom of the figure is an interphase nucleus.