Table 2 Genotypes found at six microsatellite loci in unisexual larvae from egg masses collected from a vernal pond in Mitchell Memorial Forest (MMF), Hamilton County, Ohio

From: Sex in unisexual salamanders: discovery of a new sperm donor with ancient affinities

Genotype	Microsatellite locus
	AjeD94	AjeD283	AjeD346	AjeD378	AjeD422	Atex74
Egg Mass 2
LJJ (3)	150/222/250	138/154	136/284	220/256	248/256/288	—
LJJB (1)	150/222/250	134/138/154	80^a/136/284	220/256	224^a/248/256/288	200 ^a
Egg Mass 3
LJJ (5)	150/222/250	138/154	136/284	220/256	244/256/284	—
LJJB (2)	150/222/250	138/154/198	80^a/136/284	220/256	224^a/244/256/284	200 ^a
LJJB (1)	150/222/250	138/154/166	80^a/136/284	220/256	224^a/244/256/284	192
LJJ (1)	150/226/242	134/154	136/284	220/256	244/256/284	—
LJJB (1)	150/226/242	134/154/166	80^a/136/284	220/256	208^a/244/256/284	200 ^a
Egg Mass 4
LJJ (10)	150/226/242	134/154	136/284	220/256	248/256/284	—
LJJ (3)	150/222/246	138/154	136/284	220/256	248/256/284	—
LJJB (1)	150/222/246	138/154/174^a	80^a/136/284	220/256	224^a/248/256/284	200 ^a
LJJB (1)	150/226/242	134/154/174^a	80^a/136/284	220/256	224^a/248/256/284	200 ^a
Egg Mass 5
LJJ (12)	150/222/246	138/154	136/284	220/256	244/256/284	—
LJJ (2)	150/226/242	134/154	136/284	220/256	248/256/280	—
LJJB (1)	150/222/246	138/154/202^a	80^a/136/284	220/256	208^a/248/256/284	192
LJJB (1)	150/222/246	138/154/202^a	80^a/136/284	220/256	224^a/248/256/284	192
LJJB (1)	150/222/246	134/138/154	80^a/136/284	220/256	208^a/248256/284	192
LJJB (1)	150/226/242	134/154/166	80^a/136/284	220/256	208^a/248/256/284	192
LJJB (1)	150/226/242	134/154/202^a	80^a/136/284	220/256	224^a/248/256/284	200 ^a
LJJB (1)	150/226/242	134/154/202^a	80 ^a /284	220/256	224^a/248/256/284	200 ^a
LJJB (1)	150/226/242	134/154/202^a	80^a/136/284	220/256	224^a/248/256/284	—
LJJBB (1)	150/226/242	134/154/166/174^a	80^a/136/284	220/256	208^a/224^a/248/256/284	192/200^a
LJJBB (1)	226/242	134/154/166	80^a/136/284	220/256	208^a/248/256/284	192/200^a
Egg Mass 6
LJJBB (1)	150/226/246	134/154/166/202^a	80^a/136/284	220/256	208^a/224^a/248/256/284	200 ^a
LJJB (1)	150/226/246	134/154/202^a	80^a/136/284	220/256	208^a/248/256/284	200 ^a
Egg Mass 7
LJJ (6)	150/218/226	134/154	136/284	220/256	248/256/284	—
LJJ (2)	150/218/222	134/154	136/284	220/256	248/256/284	—
LJJ (2)	150/222/242	134/154	136/284	220/256	248/256/284	—
LJJ (5)	150/222/246	134/154	136/284	220/256	248/256/284	—
LJJ (5)	150/222/250	134/154	136/284	220/256	248/256/284	—
LJJB (1)	150/222/246	134/154/178^a	80^a/136/284	220/256	208^a/248256/284	196 ^a
Egg Mass 8
LJJ (4)	150/226/242	134/154	284	220/256	248/256/284	—
LJJB (1)	150/226/242	134/154	80^a/284	220/256	208^a/248/256/284	200 ^a
Egg Mass 9
LJJ (3)	150/222/246	138/154	136/284	220/256	248/256/284	—
Egg Mass 10
LJJ (19)	150/218/246	138/154	136/284	220/256	248/256/284	—
LJJB (1)	150/218/242	138/154/182	80^a/136/284	220/256	224^a/248/256/284	200 ^a
LJJB (1)	150/218/250	138/154	80^a/136/284	220/256	224^a/248/256/284	200 ^a
LJJB (1)	150/218/246	138/154/190	80^a/136/284	220/256	224^a/248/256/284	196 ^a

Nuclear genomes include Ambystoma laterale (L), A. jeffersonianum (J) and A. barbouri (B).
The number of larvae within an egg mass that have the same multilocus genotype are in parentheses. Alleles known to be from A. laterale are in bold. Alleles assumed to be from A. barbouri are underlined.
Primers for AjeD378 only amplified A. jeffersonianum microsatellite alleles. Primers for Atex74 only amplified A. barbouri alleles. Ploidy was determined by the greatest number of alleles at any locus or from the minimum number of possible genomes. LJJBB would be a pentaploid larva having one A. laterale genome, two A. jeffersonianum genomes and two A. barbouri genomes. No amplification of microsatellite DNA alleles at a locus is —.
^aAlleles with the same mobility were found in adult specimens of A. barbouri from the same pond (see Table 1).

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Table 2 Genotypes found at six microsatellite loci in unisexual larvae from egg masses collected from a vernal pond in Mitchell Memorial Forest (MMF), Hamilton County, Ohio

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