Table 2 Summary statistics of nucleotide and haplotype variation and frequency distribution spectrum of polymorphism at analyzed genes in Scottish populations of Scots pine

From: High genetic diversity at the extreme range edge: nucleotide variation at nuclear loci in Scots pine (Pinus sylvestris L.) in Scotland

Locus

L

Nucleotide diversity

ρ b (SE)

D c

H d

Haplotype diversity

   

Non-synonymous

Silent a

   

N

H d (SD)

  

π

SNPs

π

SNP

π

πWest

πSouth

πEast

     

dhn1

1071

0.0144

8 (4)

0.0044

54 (12)

0.0203

0.0215

0.0225

0.0158

0.0113 (0.0012)

0.039

−0.285

24

0.964 (0.014)

dhn2

442

0.0074

1

0.0024

7 (0)

0.0112

0.0107

0.0110

0.0113

0.0779 (0.0379)

1.9681,2,*

0.458

11

0.888 (0.028)

dhn3

359

0.0198

10 (2)

0.0137

13 (2)

0.0291

0.0325

0.0226

0.0366

0.911

1.733

8

0.829 (0.03)

dhn7

362

0.0042

3 (1)

0.0042

4 (3)

0.0043

0.0033

0.0036

0.0061

−0.223

−2.339

6

0.711 (0.039)

dhy2PP

476

0.0101

5 (3)

0.0011

12 (2)

0.0244

0.0240

0.0223

0.0257

0.0680 (0.0228)

0.103

1.738

21

0.954 (0.015)

abaR

396

0.0048

5 (0)

0.0052

3 (2)

0.0043

0.0035

0.0048

0.0046

0.052

−2.323

10

0.755 (0.057)

a3iP2

861

0.0043

2 (2)

0.0008

12 (5)

0.0049

0.0051

0.0045

0.0054

0.0022 (0.0016)

0.360

−2.231

11

0.779 (0.052)

ccoaomt

563

0.0018

1 (1)

0.0000

5 (0)

0.0032

0.0011

0.0053

0.0038

−0.711

−0.746

5

0.348 (0.092)

chcs

330

0.0075

1 (1)

0.0008

12 (6)

0.0094

0.0066

0.0137

0.0088

−0.682

−1.267

9

0.766 (0.065)

erd3

583

0.0021

3 (3)

0.0006

7 (4)

0.0037

0.0036

0.0029

0.0046

−1.4642,*

−1.351

9

0.73 (0.052)

lp3-1

430

0.0095

1 (1)

0.0004

12 (1)

0.0137

0.0107

0.0144

0.0135

0.0195 (0.0111)

0.944

1.175

20

0.955 (0.017)

lp3-3

309

0.0370

8 (0)

0.0177

18 (1)

0.0660

0.0692

0.0683

0.0676

0.0018 (0.0011)

2.8461,2,***

1.600

19

0.949 (0.019)

Total

6182

 

48 (18)

 

159 (38)

         

Meane

515

0.0078

 

0.0031

 

0.0117

0.0111

0.0116

0.0124

0.0085 (0.0009)f

0.118

−0.494

12.18

0.789 (0.042)

  1. Abbreviations: L, length of sequence in base pairs excluding indels; Hd, haplotype diversity (standard deviation); N-number of haplotypes; SE, standard error; SNP, single-nucleotide polymorphism. Silent sites variation reported separately for west, south and east geographical groups, otherwise average values for all samples combined.
  2. π-nucleotide diversity (Nei, 1987).
  3. aSynonymous and non-coding positions.
  4. bLeast-squares estimate of recombination parameter.
  5. cD test (Tajima, 1989).
  6. dH test (Fay and Wu, 2000).
  7. eAverage values at 11 loci excluding lp3-3.
  8. fEstimates based on informative sites at all loci, excluding lp3-3; ‘—’ not estimated because of the low number of informative sites.
  9. *Statistically significant values based on coalescence simulations (1) without recombination and (2) with average recombination rate at six loci *P<0.05; ***P<0.01.