Table 2 Loci used in this study

From: DNA polymorphism in recombining and non-recombing mating-type-specific loci of the smut fungus Microbotryum

N

Loci

PCR primers

Tm, °C a

  

Forward primers

Reverse primers

 

Non-recombining A1 mating-type specific

 1

A1-pr

A1-prF TGGCATCCCTCAATGTTTCC

A1-prR CACCTTTTGATGAGAGGCCG

54.6/54.6

 2

A1-077

A1-077f CCCTCGACGATGTTCTTCTTG

A1-077r CCAGTCGAATATTTGGAACCG

53.8/53.7

 3

A1-116

A1-116f TGAGTGATGGTCGACAGGTCA

A1-116r TGACATCAGCTGAGTTCCCAGT

53.5/53.6

 4

A1-202

A1-202f ACAGGTCATTCACAACGGCT

A1-202r TCCTCGACCGTATTTATCGTG

51.8/51.9

 5

A1-210

A1-210f GGAATGCAGTGGGTCATGGT

A1-210r CGTTGCGATGACGAGGTATCT

54.3/54.2

 6

A1-276

A1-276f AGAACAGAGGCCAAGGGAGC

A1-276r CCTGAACAACCCCATCTACCC

54.8/54.7

 7

A1-281

A1-281f ACATGTTCGTAGGCGATTCG

A1-281r GTAGAGTAAGATGGCGGCCG

52.7/53.9

Non-recombining A2 mating-type specific

 8

A2-pr

A2-prF TGACGAGAGCATTCCTACCG

A2prR GAAGCGGAACTTGCCTTTCT

52.7/52.8

 9

A2-400

A2-400f TGGATGAGATGAAGTTGCGC

A2-400r CATACATTTTGGCGGGTTCA

53.5/53.3

 10

A2-441

A2-441f GNCTTGAAGCGAAGGCAAAC

A2-441r CGAAACTTACGGCTGCCATAG

54.1/54.2

 11

A2-500

A2-500f GAAGAGGGGGTTTTGACGAC

A2-500r CCCAACATGACCTCTTTCGA

52.5/52.3

 12

A2-530

A2-530f CAAGATTGAGGATAGAAGATGGATG

A2-530r GAAGTATTCGAGGTTCTTGTGGTT

53.0/52.6

Recombining

 13

paA1-134

A1-134f GGGCTTCGGAAGAAATGATC

A1-134r TCTTCCTTCCCTCAGATGGC

53.1/53.0

 14

paA1-211

A1-211f ACAGATCTCACAAAGTCGCAGA

A1-211r CACAGTGGGCTATATTTACACAAAT

53.2/53.2

 15

paA2-433

A2-433f GTCTACCGAGTCAGACTGCACA

A2-433r GGATCTATGCAGCCTTCGAC

53.5/53.4

 16

paA2-461

A2-461f CAGAAATTGGAAGCGATTGA

A2-461r ATTCCTTGCAAGTCTCGACC

53.3/53.2

 17

paA2-547

A2-547f TGGCTGCTTCTTTATGACCA

A2-547r CAAACAATTTCAATCGATGCAG

50.9/52.5

 18

Aut-932

Aut-932f ATTCGACTGCTCCCACTCTG

Aut-932r AGGAGGAGAAAGTCGATCGC

51.6/52.5

 19

Aut-935

Aut-935f TGTGGTCAGTAGAGAATGGCG

Aut-935r TCATCGTTGATCCGACCTGT

52.7/52.5

 20

Aut-948

Aut-948f GCTTGTTGTCAATCATTCATCA

Aut-948r TATGCTAATGCGGGAACAAG

50.1/50.6

  1. Abbreviation: A1-pr and A2-pr, A1- and A2-pheromone receptor genes.
  2. aAnnealing temperature for each primer; PCR was conducted at the Tm intermediate for the two primers.
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