Table 1 Pigmentation intensity among isofemale lines and collection sites

Source	Longitude	LSM	Group
Dataset A
A01	105.2	163.41	a
AA	91.4	161.39	a
DN00.2	97.5	149.36	a
DN00.4	97.5	140.96	a, b
DN01.12	97.5	128.64	b
SB	91.2	130.76	b
FP	91.1	127.97	b, c
IR	91.7	126.18	b, c, d
A09	78.9	113.44	c, d
A00	85.6	110.75	c, d
LA	90.8	109.47	c, d
A04	73.4	106.29	d
OR	83.2	98	d
Dataset B
NN	98.1	−14.53	a
DN	97.5	−15.03	a
OC	87.3	−17.76	b
MK	85.8	−18.00	b
SV	93.2	−18.57	b
II	87.5	−18.62	b
WS	82.0	−18.85	b
OR	83.2	−19.51	b

Abbreviation: LSM, least-squares mean.
For each isofemale line (‘Source’) analyzed in dataset A, the longitude of the collection site (in degrees W), the least-squares mean (LSM) from the mixed model described in Materials and methods and the pigmentation group (based on Tukey's post hoc test) are shown. A full description of each ‘Source’ is provided in Supplementary Table 1. The same metrics are shown for dataset B, except that LSMs are reported for each collection site rather than for each isofemale line and are based on a slightly different mixed model, as described in Materials and methods. Note that different pigmentation-scoring techniques were used for datasets A and B; thus, the LSM values reported for the two datasets are on different scales. Tukey's post hoc analysis was performed separately for the two datasets; thus, there is no implied relationship between groups with the same label (for example, ‘a’) in datasets A and B.

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