Table 1 Primers used in this study and the size of amplified fragments of Samia cyntia walkeri orthologues of Bombyx mori genes, and corresponding orthologous genes and/or ESTs of S. cynthia

From: Step-by-step evolution of neo-sex chromosomes in geographical populations of wild silkmoths, Samia cynthia ssp.

PCR amplification of S. c. walkeri orthologues			S. cynthia orthologues and ESTs		Bombyx mori genes
Forward primer sequence	Reverse primer sequence	Product size (bp)	Accession no.	EST (SilkBase code) ^a	Accession no.	Putative function	Chr. no.	Location in KAIKObase ^b
GGNCCNGGNGAYGARGGNGARTAYAC	GGNGGRTANACNCGRTCYTCYTCNCC	1820	AB543309^c	Not found	AB079865	Kettin	1 (Z)	6 505 696– 6 533 895
TAAAACAGTTAAACAAAAGCAGGTCAT	CTCATCTCCYTGTGCACCCCCTTGGTT	1663	AB543308	S06A01NCLL0010_M07	AB098537	BYB	1 (Z)	10 855 943– 10 857 680
GTTTGGGGAGCACATGGAGAAT	CATTTCCGTCTGCGTTCGCTTGG	ca. 2500	DQ465407	S06A01NCLL0005_L15	AB274990	3-Hydroxy-3- methylglutaryl- CoA reductase	2	916 956– 919 459
ACCGAATTAGAAGTGGTGATG	GCTTTCTTCACTCATACGTGA	ca. 2300	AF015065	Not found	AF372836	DDC	4	12 504 927– 12 521 471
GGTATCGACAAACGTACCATCG	ATGGATTTAGGGTTGTCCTCTG	1085	AF015084	I09A02NGRL0007_G07	D13338	EF-1α	5	17 106 041– 17 107 432
GTTCGCTAAGTTGTTCCTAGTG	GTGCTGGGTTCCCACGAGGACTT	921	AB059394	I09A02NGRL0003_D23	D76418	Attacin	6	18 601 317– 18 602 129
CATATTCCGCACGATGCGGG	GTTACCTAAGCCCATGTTCAT	ca. 2000	AB201280	Not found	AB104488	Chitinase	7	14 117 862– 14 119 517
GTCCAGGAGATCAACAAGACCAAG	CAGCTTCAGGAGGTTCAGCTTGTT	ca. 1800	AB543313, AB543314	S13A01NGRL0010_F15	AY769271	RpL4	11	2 852 029– 2 862 665
GGNGCNAARCTNTGYAAYATHTTYTC	TGRAARTAYTCYTTNGCYTCYTTNGA	1391	AB543310^c	Not found	AF013277	Topo II	11	8 589 427– 8 602 332
CATAAACACGACAGGAAAGTTCGGC	CTTGGTGCGCACGTATGGTTTAGT	ca. 2000	AB543311, AB543312	S06A01NCLL0020_P12	AY769287	RpL18	11	9 399 580– 9 401 164
GCTTTGCATTGCGATGCGAAACGT	ACAGTCGCTAATATCTGGCAGTCC	ca. 3000	AB048258	I10A02NGRL0003_P23	L37416	Lysozyme	12	3 312 565– 3 309 259
GCNCAYGGNTCNCARTGYGGNTTYTGYAC	TARAARTGYTCYTGNCCNCCCATNCGRCA	1985	AB543315^c	Not found	D38159	XDH I	12	8 502 165– 8 493 509
GTTGCCGCGTTGGTAGTAGACAA	CCAGACTCGTCGTACTCCTGTTT	1169	AB543316	I10A02NGRL0006_L21	U49644	Cytoplasmic actin A4	17	2 936 956– 2 938 173
ATTCAGCGTACAATTCTTATGATGGG	CCAATGATCAAATACGCATTGTGGGAA	1113	AB543317	S06A01NCLL0007_L10	DQ443396	eEF-2	19	12 802 871– 12 811 573
AAAATGGGCATGCTACCGCGTGG	GCTCATCAACATGACGTCTTTGTA	ca. 2300	AB288051	S13A02NGRL0010_N13	X12978	SP1	23	16 906 448– 16 911 216
GGGTAAACAATGGCGTCTAAG	GCTCTGCAGCCGTAGTATCCC	ca. 3000	AB220992	I09A02NGRL0003_K24	AY515321	Hemolin	24	13 879 059– 13 884 127

Abbreviations: BYB, Y-box protein; DDC, DOPA decarboxylase; DOP-PCR, degenerate oligonucleotide-primed PCR; EF-1α, elongation factor 1α; eEF-2, translation elongation factor 2; EST, expressed sequence tag; RpL4, ribosomal protein L4; RpL18, ribosomal protein L18; SP1, sex-specific storage-protein 1; Topo II, topoisomerase II; XDH I, xanthine dehydrogenase I.
^aESTs showing the highest score were taken from http://silkbase.ab.a.u-tokyo.ac.jp/cgi-bin/index.cgi.
^bInferred from http://sgp.dna.affrc.go.jp/KAIKObase/.
^cAn orthlogous sequence was obtained using DOP-PCR.

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Table 1 Primers used in this study and the size of amplified fragments of Samia cyntia walkeri orthologues of Bombyx mori genes, and corresponding orthologous genes and/or ESTs of S. cynthia

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