Figure 1 | Heredity

Figure 1

From: Isolation and characterization of a female-specific DNA marker in the giant freshwater prawn Macrobrachium rosenbergii

Figure 1

Amplified fragment length polymorphism (AFLP) screening for sex-specific genomic sequences in M. rosenbergii and PCR of genomic DNA extracted from mature M. rosenbergii. (a) AFLP profiles of three male and three female mature M. rosenbergii prawns obtained with a combination of Eco-NN- and Mse-NN-selective primers (‘N’ represents a randomly picked nucleotide). Representative monomorphic (M), polymorphic (P) and male-specific (MS) sequences are indicated by arrows. (b) The female-specific genomic sequence (FS) is amplified in all mature female specimens and is absent in all mature male specimens, as shown by PCR. Positive control (PC), which was derived from an AFLP monomorphic band, is present in all specimens. Primer sequences are available upon request.

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