Figure 2
Schematic overview of the original sex-specific sequence found by amplified fragment length polymorphism (AFLP) and the further upstream and downstream sequence features obtained by genome walking and sequence characterization. (a) The ∼3-kbp sequence obtained by AFLP and genome walking. Seven tandem repeats of 88 bp, each starting with a 12-bp palindrome, are indicated by striped rectangles, as are three 11-bp inverted repeats (indicated by triangles). (b) A 340-bp sequence within the ∼3-kbp sequence obtained by genomic walking in the area surrounding the original AFLP product was amplified both in male and female prawns. In this 340-bp sequence, two female-specific SNPs were identified (
), which enabled the female-specific amplification of the SCAR marker. An insertion of three nucleotides (
) was identified in all male sequences, but only in some of the female ones. Primers that include (
) or bypass (
) the insertion were designed. A full color version of this figure is available at the Heredity journal online.
