Figure 1
Overview of the design of the productivity assay. A single female from each of the stock and two of the mutant populations were mated to their own males and then placed together for oviposition in a single ‘mixed’ vial containing either the ancestral (cornmeal) or the novel (corn-flour) food. From this vial, four virgin females were collected, two stock and one of each of the two mutants, and used to set up four productivity vials containing the same food as that on which the female was raised. To each vial was added a single stock male, raised in cornmeal, with all four males collected from the same vial. Approximately 150 replicates of this design were set up for each pair of mutants.
