Table 2 Effects of nine separate mutations on the productivity of mutant (W̄_m) and stock (W̄_s) females, and the resulting selective coefficient (s_p; a negative value corresponds to a deleterious allele) when assayed in two different environments

Mutation	Ancestral (cornmeal) environment				Novel (corn-flour) environment
	W̄_m	W̄_m	s_p	P	W̄_m	W̄_m	s_p	P
brown	79.5	76.0	0.045	0.385	50.9	53.3	−0.045	0.316
cinnabar	82.0	76.0	0.079	0.101	51.3	53.3	−0.037	0.404
claret	60.7	70.6	−0.140	<0.001^*	35.1	35.0	0.001	0.991
eyeless	57.5	68.8	−0.164	<0.001^*	27.8	43.5	−0.361	<0.001^*
forked	73.9	70.6	0.047	0.223	32.6	35.0	−0.070	0.265
plexus	69.4	72.8	−0.047	0.292	41.2	38.0	0.084	0.133
sepia	47.5	71.8	−0.338	<0.001^*	27.4	36.5	−0.249	<0.001^*
white	63.8	71.8	−0.112	0.066	33.2	36.5	−0.089	0.187
yellow	74.5	72.8	0.023	0.642	41.3	38.0	0.086	0.142
Mean (±s.e)	67.6 (3.7)	72.4 (0.8)	−0.067 (0.045)		37.9 (3.0)	41.0 (2.5)	−0.076 (0.049)

Significance (P) was evaluated by one-sample t-test (t) to determine whether the difference in productivity (mutant−stock) differed from 0 (the null hypothesis of equal productivity of stock and mutant females), treating vials as replicates. Degrees-of-freedom vary depending on the number of replicate vials and range from 111 to 152. The asterisks denote P⩽0.05.

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