Figure 2 | Heredity

Figure 2

From: Contrasting the distribution of phenotypic and molecular variation in the freshwater snail Biomphalaria pfeifferi, the intermediate host of Schistosoma mansoni

Figure 2

Schematic diagram of the experimental protocol conducted in the seven populations studied (more details in text). Gi refers to generation. Seven to twenty field-caught G0 individuals per population were isolated. Sixty G1 offspring were chosen at random (from 3 to 10 G1 depending on sample size in natural populations), and set in three groups of 20 individuals on week 3. At week 6, 10 individuals were sampled per group (30 G1 individuals per population), and isolated for 25 weeks (week 7–31) during which fecundity, growth and survival were monitored. At week 18, egg capsules were collected to monitor the hatching rate of G2 individuals. One week after hatching (10–13 days), two G2 were randomly chosen per G1 snail, and they were isolated in boxes for 17 weeks and monitored up to sexual maturity in order to quantify some life-history traits (size and fecundity).

Back to article page