Table 5 Length, G+C content and variation in Gibbs free energy of the secondary structure of ITS and 5.8S rDNA sequences in Eyprepocnemis plorans
Origin of DNA sequences | N | All regions | ITS1 | 5.8S | ITS2 | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Length (min–max) | %G+C | Length (min–max) | %G+C | ΔG (kcal mol−1) | Length (min–max) | %G+C | ΔG (kcal mol−1) | Length (min–max) | %G+C | ΔG (kcal mol−1) | ||
Genomic (0B) | 10 | 930.7 (912–948) | 56.5 | 368.4 (347–386) | 60.8 | −176.0 | 158.0 (158–158) | 58.9 | −57.5 | 178.3 (178–181) | 64.0 | −91.1 |
Genomic (+B) | 13 | 942.2 (928–953) | 57.3 | 380.1 (366–390) | 61.1 | −180.8 | 158.0 (158–158) | 59.1 | −58.1 | 178.2 (178–179) | 64.0 | −90.4 |
M8 chromosome | 7 | 908.7 (908–909) | 54.9 | 346.9 (346–347) | 60.2 | −160.0 | 157.9 (157–158) | 58.6 | −57.6 | 178.0 (178–178) | 64.5 | −92.6 |
S9 chromosome | 6 | 909.0 (909–909) | 57.8 | 347.0 (347–347) | 60.3 | −160.7 | 158.0 (158–158) | 58.9 | −57.8 | 178.0 (178–178) | 64.8 | −91.8 |
S10 chromosome | 5 | 909.0 (909–909) | 55.0 | 384.2 (359–390) | 61.7 | −160.0 | 157.9 (157–158) | 58.7 | −57.6 | 178.6 (177–179) | 64.2 | −88.6 |
S11 chromosome | 6 | 939.7 (934–941) | 56.9 | 379.0 (379–379) | 60.8 | −179.9 | 158.0 (158–158) | 59.0 | −57.9 | 176.3 (168–178) | 63.4 | −85.0 |
X chromosome | 11 | 927.7 (926–928) | 56.1 | 365.8 (364–366) | 59.8 | −167.9 | 158.0 (158–158) | 59.0 | −57.8 | 178.0 (178–178) | 64.8 | −93.3 |
B24 chromosome | 17 | 950.9 (928–953) | 58.3 | 388.4 (366–390) | 62.1 | −184.4 | 157.9 (157–158) | 58.7 | −56.9 | 178.8 (177–179) | 64.3 | −91.1 |
Weighted mean | ||||||||||||
E. plorans | 75 | 932.3 | 56.9 | 372.8 | 61.0 | −174.1 | 158.0 | 58.9 | −57.6 | 178.2 | 64.3 | −90.8 |
C. parallelus | — | 899.0 | 58.1 | 367.4 | 59.4 | −137.6 | 158.0 | 58.8 | −58.8 | 184.0 | 66.8 | −102.9 |
L. migratoria | — | 912.0 | 59.3 | 379.1 | 61.6 | −134.7 | 158.0 | 58.8 | −58.8 | 200.0 | 68.5 | −112.8 |