Table 2 NZSL MHC DRB alleles and frequency in NZSL live pup population as derived from Sanger sequencing and Phase reconstruction

From: Extensive variation at MHC DRB in the New Zealand sea lion (Phocarctos hookeri) provides evidence for balancing selection

Allele number

Alleles—bp position consensus

Count

Frequency

 

58

59

61

88

148

151

161

168

170

  

1

T

T

G

C

C

C

C

T

C

1

0.57

2/5

T

T

G

C

C

C

G

T

A

1

0.57

3/4

T

T

G

C

C

A

G

T

A

43

24.71

6

T

T

G

C

A

C

C

T

C

2

1.15

7

T

T

G

C

T

A

C

T

C

4

2.3

8

T

T

G

T

C

C

G

C

A

3

1.72

9

T

T

G

T

C

A

A

C

A

1

0.57

10

T

T

G

T

C

A

G

C

A

21

12.07

11

T

T

G

T

A

C

C

T

C

1

0.57

12

T

A

G

C

T

A

C

T

C

3

1.72

13

A

T

T

C

A

C

C

T

C

1

0.57

14

A

T

T

T

C

C

C

T

A

2

1.15

15

A

T

T

T

C

C

G

T

A

6

3.45

16

A

T

T

T

C

C

G

T

C

1

0.57

17

A

T

T

T

C

A

G

T

A

1

0.57

18

A

T

T

T

A

C

C

T

A

1

0.57

19

A

T

T

T

A

C

C

T

C

2

1.15

20

A

T

T

T

A

C

G

T

A

2

1.15

21

A

T

T

T

A

C

G

T

C

1

0.57

22

A

A

G

T

C

C

G

C

A

1

0.57

23

A

A

T

T

C

C

C

T

C

2

1.15

24

A

A

T

T

C

C

G

T

A

1

0.57

25

A

A

T

T

C

C

G

T

C

4

2.3

26

A

A

T

T

A

C

C

T

C

67

38.51

27

A

A

T

T

A

C

G

T

C

1

0.57

28

A

A

T

T

G

C

C

T

C

1

0.57

  1. The most frequent alleles and those used in further analyses are shaded grey. Base pair (bp) consensus positions are arbitrarily calculated from the start position of the amplified PCR fragment in NZSL DNA. Alleles 2 and 5, and 3 and 4, were synonymous at the amino-acid level, forming two distinct alleles by protein sequence. Here, frequencies are combined, and the most common nucleotide sequence is listed.
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