Figure 3

Schematic diagram of BmAntp sequences of the wild type (Dazao) and mutant (Wes/Wes). (a) BmAntp transcripts amplified by P1 in Dazao and Wes/Wes. The Bmactin 3 was used as an internal control. (b) Comparison of the structure of BmAntp between Dazao and Wes/Wes. White boxes, exons; diagonal lines, introns; coarse horizontal lines, untranslated regions; black box, the insertion; gray boxes, target site duplication (TSD); gray arrows below Type 1, the primer set Antp-C; and black arrows below Type 2, the primer set Antp-M. (c) Diagram of a LINE-like element with a 5′ truncation insertion in exon 3 of the BmAntp gene. The BmAntp gene spans 1 551 bp and contains three exons. The 1570 bp insertion is flanked by a 15-base TSD of BmAntp cDNA sequence (nucleotides 1 076–1 090). Nucleotides 3 533–5061 represent the BMC1 element.